Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
Artikel i vetenskaplig tidskrift, 2017

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

AC susceptometry

Magnetic nanoparticles

RCA coil

Magnetic multi-core particles

Differential induction coil

Biodetection

Författare

Fredrik Ahrentorp

Jakob Blomgren

Christian Jonasson

Anna Sarwe

Sobhan Sepehri

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Emil Eriksson

Chalmers, Mikroteknologi och nanovetenskap (MC2)

Alexei Kalaboukhov

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Aldo Jesorka

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Dag Winkler

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Justin Schneiderman

University of Gothenburg and MedTech West

Mats Nilsson

Stockholms universitet

Jan Albert

Karolinska universitetssjukhuset

Teresa Zardán Gómez de la Torre

Uppsala universitet

Maria Strømme

Uppsala universitet

Christer Johansson

Journal of Magnetism and Magnetic Materials

0304-8853 (ISSN)

Vol. 427 14-18

Ämneskategorier

Analytisk kemi

DOI

10.1016/j.jmmm.2016.10.041

Mer information

Senast uppdaterat

2018-03-01