Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
Artikel i vetenskaplig tidskrift, 2017
We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.
Biodetection
Magnetic multi-core particles
AC susceptometry
Magnetic nanoparticles
RCA coil
Differential induction coil
Författare
Fredrik Ahrentorp
Acreo Swedish ICT AB
Jakob Blomgren
Acreo Swedish ICT AB
Christian Jonasson
Acreo Swedish ICT AB
Anna Sarwe
Acreo Swedish ICT AB
Sobhan Sepehri
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Emil Eriksson
Chalmers, Mikroteknologi och nanovetenskap
Alexei Kalaboukhov
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Aldo Jesorka
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Dag Winkler
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Justin Schneiderman
Göteborgs universitet
MedTech West
Mats Nilsson
Stockholms universitet
Jan Albert
Karolinska universitetssjukhuset
Teresa Zardán Gómez de la Torre
Uppsala universitet
Maria Strømme
Uppsala universitet
Christer Johansson
Acreo Swedish ICT AB
Journal of Magnetism and Magnetic Materials
0304-8853 (ISSN)
Vol. 427 14-18Nanometrology Standardization Methods for Magnetic Nanoparticles (NANOMAG)
Europeiska kommissionen (EU) (EC/FP7/604448), 2013-11-01 -- 2017-10-31.
Ämneskategorier
Analytisk kemi
Annan fysik
DOI
10.1016/j.jmmm.2016.10.041