The direction of human mesenchymal stem cells into the chondrogenic lineage is influenced by the features of hydrogel carriers
Artikel i vetenskaplig tidskrift, 2017

Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell- survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix((R)) or Hydromatrix((R)) and potential effects of stimulation with growth hormone (GH). hMSCs/hydrogel cultures were investigated for cell-viability, attachment, gene expressionof chondrogenic markers SOX9, COL2A1, ACAN and accumulation of extracellular matrix (ECM). In both hydrogel types, hMSCs were viable for 28 days, expressed integrin beta 1 which indicates adhesion of hMSCs. Differentiation was observed into chondrocyte-like cells, in a higher extent in hMSCs/Hydromatrix((R)) cultures when compared to hMSCs/Puramatrix ((R)) hydrogel cultures. Gene expression analyses of chondrogenic markers verified results. hMSCs/hydrogel cultures stimulated with GH displayed no significant effects on chondrogenesis. In conclusion, both hydrogels, especially Hydromatrix((R)) was demonstrated as a promising cell carrier in vitro for hMSCs, when directed into chondrogenesis. This knowledge could be useful in biological approaches for regeneration of degenerated human IVDs.

Intervertebral disc

growth-factors

1964

Cell carrier

low-back-pain

in-vitro

intervertebral disc degeneration

v4

repair

Hydrogels

applications

Cell Biology

therapy

ott j. e.

transplantation

Anatomy & Morphology

tissue-engineering

p73

Human mesenchymal stem cells

vivo

model

histochemie

Disc degeneration

Författare

A. Hansson

Sahlgrenska akademin

Chalmers University of Technology

Anna Wenger

Chalmers, Biologi och bioteknik

H. B. Henriksson

Sahlgrenska universitetssjukhuset

Sahlgrenska akademin

S. Li

Göteborgs universitet

B. R. Johansson

Göteborgs universitet

H. Brisby

Sahlgrenska akademin

Sahlgrenska universitetssjukhuset

Tissue and Cell

0040-8166 (ISSN)

Vol. 49 35-44

Ämneskategorier

Cell- och molekylärbiologi

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.tice.2016.12.004