Autophagy induction regulates influenza virus replication in a time-dependent manner
Artikel i vetenskaplig tidskrift, 2017

Purpose. Autophagy plays a key role in host defence responses against microbial infections by promoting degradation of pathogens and participating in acquired immunity. The interaction between autophagy and viruses is complex, and this pathway is hijacked by several viruses. Influenza virus (IV) interferes with autophagy through its replication and increases the accumulation of autophagosomes by blocking lysosome fusion. Thus, autophagy could be an effective area for antiviral research. Methodology. In this study, we evaluated the effect of autophagy on IV replication. Two cell lines were transfected with Beclin-1 expression plasmid before (prophylactic approach) and after (therapeutic approach) IV inoculation. Results/Key findings. Beclin-1 overexpression in the cells infected by virus induced autophagy to 26 %. The log10 haemagglutinin titre and TCID50 (tissue culture infective dose giving 50% infection) of replicating virus were measured at 24 and 48 h post-infection. In the prophylactic approach, the virus titre was enhanced significantly at 24 h post-infection (P?0.01), but it was not significantly different from the control at 48 h post-infection. In contrast, the therapeutic approach of autophagy induction inhibited the virus replication at 24 and 48 h post-infection. Additionally, we showed that inhibition of autophagy using 3-methyladenine reduced viral replication. Conclusion. This study revealed that the virus (H1N1) titre was controlled in a time-dependent manner following autophagy induction in host cells. Manipulation of autophagy during the IV life cycle can be targeted both for antiviral aims and for increasing viral yield for virus production.

Autophagy

Replication

Influenza virus

Beclin-1

Författare

Neda Feizi

Pasteur Institute of Iran

Parvaneh Mehrbod

Pasteur Institute of Iran

Bizhan Romani

University of Alberta

Ahvaz, Jundishapur University of Medical Sciences

Hoorieh Soleimanjahi

Tarbiat Modares University

Taravat Bamdad

Tarbiat Modares University

Amir Feizi

Chalmers, Biologi och bioteknik, Systembiologi

Ehsan Ollah Jazaeri

Pasteur Institute of Iran

Hadiseh Shokouhi Targhi

Pasteur Institute of Iran

Maryam Saleh

Pasteur Institute of Iran

Abbas Jamali

Pasteur Institute of Iran

Fatemeh Fotouhi

Pasteur Institute of Iran

Reza Nasrollahi Nargesabad

Pasteur Institute of Iran

Asghar Abdoli

Pasteur Institute of Iran

Journal of Medical Microbiology

0022-2615 (ISSN)

Vol. 66 536-541

Ämneskategorier

Immunologi

Infektionsmedicin

Mikrobiologi inom det medicinska området

DOI

10.1099/jmm.0.000455

PubMed

28463668