Exploring the potential of Saccharomyces cerevisiae for biopharmaceutical protein production
Artikel i vetenskaplig tidskrift, 2017

Production of recombinant proteins by yeast plays a vital role in the biopharmaceutical industry. It is therefore desirable to develop yeast platform strains for over-production of various biopharmaceutical proteins, but this requires fundamental knowledge of the cellular machinery, especially the protein secretory pathway. Integrated analyses of multi-omics datasets can provide comprehensive understanding of cellular function, and can enable systems biology-driven and mathematical model-guided strain engineering. Rational engineering and introduction of trackable genetic modifications using synthetic biology tools, coupled with high-throughput screening are, however, also efficient approaches to relieve bottlenecks hindering high-level protein production. Here we review advances in systems biology and metabolic engineering of yeast for improving recombinant protein production.

Machinery

Metabolic engineering

Throughput

Functions

Yeast

Recombinant proteins

Biology

Författare

Guokun Wang

Chalmers, Biologi och bioteknik, Systembiologi

Mingtao Huang

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Current Opinion in Biotechnology

0958-1669 (ISSN)

Vol. 48 77-84

Ämneskategorier

Biokemi och molekylärbiologi

Programvaruteknik

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1016/j.copbio.2017.03.017

Mer information

Skapat

2017-10-07