Review of combined isotopic and optical nanoscopy
Reviewartikel, 2017

Investigating the detailed substructure of the cell is beyond the ability of conventional optical microscopy. Electron microscopy, therefore, has been the only option for such studies for several decades. The recent implementation of several super-resolution optical microscopy techniques has rendered the investigation of cellular substructure easier and more efficient. Nevertheless, optical microscopy only provides an image of the present structure of the cell, without any information on its long-Temporal changes. These can be investigated by combining super-resolution optics with a nonoptical imaging technique, nanoscale secondary ion mass spectrometry, which investigates the isotopic composition of the samples. The resulting technique, combined isotopic and optical nanoscopy, enables the investigation of both the structure and the "history" of the cellular elements. The age and the turnover of cellular organelles can be read by isotopic imaging, while the structure can be analyzed by optical (fluorescence) approaches. We present these technologies, and we discuss their implementation for the study of biological samples. We conclude that, albeit complex, this type of technology is reliable enough for mass application to cell biology.


stimulated emission depletion microscopy

photoactivated light microscopy

protein turnover

secondary ion mass spectrometry

super-resolution microscopy

stochastic optical reconstruction microscopy

correlated imaging


Katharina N. Richter

Georg-August-Universität Göttingen

Silvio O. Rizzoli

Georg-August-Universität Göttingen

Sebastian Jähne

Georg-August-Universität Göttingen


Angela Vogts

Institut fur Ostseeforschung Warnemunde

Jelena Lovric

Chalmers, Kemi och kemiteknik, Kemi och biokemi


2329423x (ISSN) 23294248 (eISSN)

Vol. 4 2 020901- 020901


Biologiska vetenskaper





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