Insights into the anticancer properties of the first antimicrobial peptide from Archaea
Artikel i vetenskaplig tidskrift, 2017

Background: The peptide VLL-28, identified in the sequence of an archaeal protein, the transcription factor Stf76 from Sulfolobus islandicus, was previously identified and characterized as an antimicrobial peptide, possessing a broad-spectrum antibacterial activity. Methods: Through a combined approach of NMR and Circular Dichroism spectroscopy, Dynamic Light Scattering, confocal microscopy and cell viability assays, the interaction of VLL-28 with the membranes of both parental and malignant cell lines has been characterized and peptide mechanism of action has been studied. Results: It is here demonstrated that VLL-28 selectively exerts cytotoxic activity against murine and human tumor cells. By means of structural methodologies, VLL-28 interaction with the membranes has been proven and the binding residues have been identified. Confocal microscopy data show that VLL-28 is internalized only into tumor cells. Finally, it is shown that cell death is mainly caused by a time-dependent activation of apoptotic pathways. Conclusions: VLL-28, deriving from the archaeal kingdom, is here found to be endowed with selective cytotoxic activity towards both murine and human cancer cells and consequently can be classified as an ACP.

Peptide-membrane interactions

Antimicrobial peptide

Archaea

Sulfolobus

Anticancer peptide

Författare

R. Gaglione

Universita degli Studi di Napoli Federico II

L. Pirone

Consiglio Nazionale delle Ricerche

B. Farina

Advanced Accelerator Applications

Consiglio Nazionale delle Ricerche

Salvatore Fusco

Chalmers, Biologi och bioteknik, Industriell bioteknik

G. Smaldone

IRCCS Fondazione SDN

Martina Aulitto

Universita degli Studi di Napoli Federico II

E. Dell'Olmo

Universita degli Studi di Napoli Federico II

E. Roscetto

Med. School University Federico II

A. Del Gatto

Consiglio Nazionale delle Ricerche

Universita degli Studi di Napoli Federico II

R. Fattorusso

University of Campania Luigi Vanvitelli

Universita degli Studi di Napoli Federico II

E. Notomista

Universita degli Studi di Napoli Federico II

L. Zaccaro

Consiglio Nazionale delle Ricerche

Universita degli Studi di Napoli Federico II

A. Arciello

Universita degli Studi di Napoli Federico II

Istituto Nazionale di Biostrutture e Biosistemi

Emilia Pedone

Universita degli Studi di Napoli Federico II

Consiglio Nazionale delle Ricerche

Patrizia Contursi

Universita degli Studi di Napoli Federico II

Biochimica et Biophysica Acta - General Subjects

0304-4165 (ISSN)

Vol. 1861 9 2155-2164

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/j.bbagen.2017.06.009