Insights into the anticancer properties of the first antimicrobial peptide from Archaea
Artikel i vetenskaplig tidskrift, 2017

Background: The peptide VLL-28, identified in the sequence of an archaeal protein, the transcription factor Stf76 from Sulfolobus islandicus, was previously identified and characterized as an antimicrobial peptide, possessing a broad-spectrum antibacterial activity. Methods: Through a combined approach of NMR and Circular Dichroism spectroscopy, Dynamic Light Scattering, confocal microscopy and cell viability assays, the interaction of VLL-28 with the membranes of both parental and malignant cell lines has been characterized and peptide mechanism of action has been studied. Results: It is here demonstrated that VLL-28 selectively exerts cytotoxic activity against murine and human tumor cells. By means of structural methodologies, VLL-28 interaction with the membranes has been proven and the binding residues have been identified. Confocal microscopy data show that VLL-28 is internalized only into tumor cells. Finally, it is shown that cell death is mainly caused by a time-dependent activation of apoptotic pathways. Conclusions: VLL-28, deriving from the archaeal kingdom, is here found to be endowed with selective cytotoxic activity towards both murine and human cancer cells and consequently can be classified as an ACP.

Antimicrobial peptide

Sulfolobus

Peptide-membrane interactions

Archaea

Anticancer peptide

Författare

R. Gaglione

Universita degli Studi di Napoli Federico II

L. Pirone

Consiglo Nazionale Delle Richerche

B. Farina

Consiglo Nazionale Delle Richerche

Advanced Accelerator Applications

Salvatore Fusco

Chalmers, Biologi och bioteknik, Industriell bioteknik

G. Smaldone

IRCCS Fondazione SDN

Martina Aulitto

Universita degli Studi di Napoli Federico II

E. Dell'Olmo

Universita degli Studi di Napoli Federico II

E. Roscetto

Universita degli Studi di Napoli Federico II

A. Del Gatto

Consiglo Nazionale Delle Richerche

Universita degli Studi di Napoli Federico II

R. Fattorusso

Universita degli Studi di Napoli Federico II

University of Campania Luigi Vanvitelli

E. Notomista

Universita degli Studi di Napoli Federico II

L. Zaccaro

Consiglo Nazionale Delle Richerche

Universita degli Studi di Napoli Federico II

A. Arciello

Istituto Nazionale di Biostrutture e Biosistemi

Universita degli Studi di Napoli Federico II

Emilia Pedone

Consiglo Nazionale Delle Richerche

Universita degli Studi di Napoli Federico II

Patrizia Contursi

Universita degli Studi di Napoli Federico II

Biochimica et Biophysica Acta - General Subjects

0304-4165 (ISSN)

Vol. 1861 9 2155-2164

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/j.bbagen.2017.06.009

Mer information

Senast uppdaterat

2020-11-13