Engineering Robustness of Microbial Cell Factories
Artikel i vetenskaplig tidskrift, 2017

Metabolic engineering and synthetic biology offer great prospects in developing microbial cell factories capable of converting renewable feedstocks into fuels, chemicals, food ingredients, and pharmaceuticals. However, prohibitively low production rate and mass concentration remain the major hurdles in industrial processes even though the biosynthetic pathways are comprehensively optimized. These limitations are caused by a variety of factors unamenable for host cell survival, such as harsh industrial conditions, fermentation inhibitors from biomass hydrolysates, and toxic compounds including metabolic intermediates and valuable target products. Therefore, engineered microbes with robust phenotypes is essential for achieving higher yield and productivity. In this review, the recent advances in engineering robustness and tolerance of cell factories is described to cope with these issues and briefly introduce novel strategies with great potential to enhance the robustness of cell factories, including metabolic pathway balancing, transporter engineering, and adaptive laboratory evolution. This review also highlights the integration of advanced systems and synthetic biology principles toward engineering the harmony of overall cell function, more than the specific pathways or enzymes.

adaptive laboratory evolution

dynamic metabolic engineering

systems and synthetic biology

robustness engineering

microbial cell factories

Författare

Z. W. Gong

Dalian Institute of Chemical Physics Chinese Academy of Sciences

Wuhan University of Science and Technology

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Yongjin Zhou

Dalian Institute of Chemical Physics Chinese Academy of Sciences

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)

Vol. 12 10

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1002/biot.201700014