Enabling the synthesis of medium chain alkanes and 1-alkenes in yeast
Artikel i vetenskaplig tidskrift, 2017

Microbial synthesis of medium chain aliphatic hydrocarbons, attractive drop-in molecules to gasoline and jet fuels, is a promising way to reduce our reliance on petroleum-based fuels. In this study, we enabled the synthesis of straight chain hydrocarbons (C7–C13) by yeast Saccharomyces cerevisiae through engineering fatty acid synthases to control the chain length of fatty acids and introducing heterologous pathways for alkane or 1-alkene synthesis. We carried out enzyme engineering/screening of the fatty aldehyde deformylating oxygenase (ADO), and compartmentalization of the alkane biosynthesis pathway into peroxisomes to improve alkane production. The two-step synthesis of alkanes was found to be inefficient due to the formation of alcohols derived from aldehyde intermediates. Alternatively, the drain of aldehyde intermediates could be circumvented by introducing a one-step decarboxylation of fatty acids to 1-alkenes, which could be synthesized at a level of 3 mg/L, 25-fold higher than that of alkanes produced via aldehydes.

1-Alkenes

Saccharomyces cerevisiae

Medium-chain fatty acids

Alkanes

Författare

Zhu Zhiwei

Chalmers, Biologi och bioteknik, Systembiologi

Yongjin Zhou

Chalmers, Biologi och bioteknik, Systembiologi

Min-Kyoung Kang

Chalmers, Biologi och bioteknik, Systembiologi

Chalmers, Biologi och bioteknik

Anastasia Krivoruchko

Chalmers, Biologi och bioteknik, Systembiologi

Nicolaas Buijs

Chalmers, Biologi och bioteknik, Systembiologi

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 44 NOV 81-88

Drivkrafter

Hållbar utveckling

Ämneskategorier

Industriell bioteknik

Infrastruktur

Chalmers infrastruktur för masspektrometri

Fundament

Grundläggande vetenskaper

Styrkeområden

Livsvetenskaper och teknik

DOI

10.1016/j.ymben.2017.09.007