Molecular-Level Profiling of Human Serum Transferrin Protein through Assessment of Nanopore-Based Electrical and Chemical Responsiveness
Artikel i vetenskaplig tidskrift, 2019

In this study, we investigated the voltage and pH responsiveness of human serum transferrin (hSTf) protein using silicon nitride (SixNy) nanopores. The Fe(III)-rich holo form of hSTf was dominant when pH > pI, while the Fe(III)-free apo form was dominant when pH < pI. The translocations of hSTf were purely in an electrophoretic sense, thus depended on its pI and the solution pH. With increasing voltage, voltage driven protein unfolding became prominent which was indicated by the trends associated with change in conductance, due to hSTf translocation, and in the excluded electrolyte volume. Additionally, analysis of the translocation events of the pure apo form of hSTf showed a clear difference in the event population compared to that of the holo form. The results obtained demonstrate the successful application of nanopore devices to distinguish between the holo and apo forms of hSTf in a mixture and to analyze its folding and unfolding phenomenon over a range of pH and applied voltages.

protein conformation


excluded volume

controlled dielectric breakdown


human serum transferrin


Jugal Saharia

Southern Methodist University

Y. M. Nuwan D. Y. Bandara

Southern Methodist University

Gaurav Goyal

Chalmers, Biologi och bioteknik, Kemisk biologi

Jung Soo Lee

Southern Methodist University

Buddini Iroshika Karawdeniya

Southern Methodist University

Min Jun Kim

Southern Methodist University

ACS Nano

1936-0851 (ISSN) 1936-086X (eISSN)

Vol. 13 4 4246-4254


Biokemi och molekylärbiologi

Annan medicinsk grundvetenskap






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