NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1)
Artikel i vetenskaplig tidskrift, 2004

A strain of Saccharomyces cerevisiae lacking the GPD2 gene, encoding one of the glycerol-3-phosphate dehydrogenases, grows slowly under anaerobic conditions, due to reductive stress caused by the accumulation of cytoplasmic NADH. We used 2D-PAGE to study the effect on global protein expression of reductive stress in the anaerobically grown gpd2Delta strain. The most striking response was a strongly elevated expression of Tdh1p, the minor isoform of glyceraldehyde-3-phosphate dehydrogenase. This increased expression could be reversed by the addition of acetoin, a NADH-specific redox sink, which furthermore largely restored anaerobic growth of the gpd2Delta strain. Additional deletion of the TDH1 gene (but not of TDH2 or TDH3) improved anaerobic growth of the gpd2Delta strain. We therefore propose that TDH1 has properties not displayed by the other TDH isogenes and that its expression is regulated by reductive stress caused by an excess of cytoplasmic NADH.

redox balances

yeast-cells

adaptation

redox

mutants

2d-page

gpd2

reductive stress

protein

glycerol formation

yeast

metabolism

gts1 gene-product

anaerobic growth

Författare

Hadi Valadi

Chalmers, Institutionen för kemi och biovetenskap

Åsa Valadi

Göteborgs universitet

R. Ansell

Natl. Laboratory of Forensic Science

Lena Gustafsson

Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik

Lennart Adler

Göteborgs universitet

Joakim Norbeck

Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik

Anders Blomberg

Göteborgs universitet

Current Genetics

0172-8083 (ISSN) 1432-0983 (eISSN)

Vol. 45 2 90-95

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1007/s00294-003-0469-1