NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1)
Artikel i vetenskaplig tidskrift, 2004
A strain of Saccharomyces cerevisiae lacking the GPD2 gene, encoding one of the glycerol-3-phosphate dehydrogenases, grows slowly under anaerobic conditions, due to reductive stress caused by the accumulation of cytoplasmic NADH. We used 2D-PAGE to study the effect on global protein expression of reductive stress in the anaerobically grown gpd2Delta strain. The most striking response was a strongly elevated expression of Tdh1p, the minor isoform of glyceraldehyde-3-phosphate dehydrogenase. This increased expression could be reversed by the addition of acetoin, a NADH-specific redox sink, which furthermore largely restored anaerobic growth of the gpd2Delta strain. Additional deletion of the TDH1 gene (but not of TDH2 or TDH3) improved anaerobic growth of the gpd2Delta strain. We therefore propose that TDH1 has properties not displayed by the other TDH isogenes and that its expression is regulated by reductive stress caused by an excess of cytoplasmic NADH.
mutants
redox balances
yeast
2d-page
yeast-cells
gts1 gene-product
glycerol formation
metabolism
gpd2
adaptation
reductive stress
protein
anaerobic growth
redox
Författare
Hadi Valadi
Chalmers, Institutionen för kemi och biovetenskap
Åsa Valadi
Göteborgs universitet
R. Ansell
Statens kriminaltekniska laboratorium (SKL)
Lena Gustafsson
Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik
Lennart Adler
Göteborgs universitet
Joakim Norbeck
Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik
Anders Blomberg
Göteborgs universitet
Current Genetics
0172-8083 (ISSN) 1432-0983 (eISSN)
Vol. 45 2 90-95Ämneskategorier
Biokemi och molekylärbiologi
DOI
10.1007/s00294-003-0469-1