Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid
Artikel i vetenskaplig tidskrift, 2016

Background: 5-Aminovaleric acid (5AVA) is an important five-carbon platform chemical that can be used for the synthesis of polymers and other chemicals of industrial interest. Enzymatic conversion of l-lysine to 5AVA has been achieved by employing lysine 2-monooxygenase encoded by the davB gene and 5-aminovaleramidase encoded by the davA gene. Additionally, a recombinant Escherichia coli strain expressing the davB and davA genes has been developed for bioconversion of l-lysine to 5AVA. To use glucose and xylose derived from lignocellulosic biomass as substrates, rather than l-lysine as a substrate, we previously examined direct fermentative production of 5AVA from glucose by metabolically engineered E. coli strains. However, the yield and productivity of 5AVA achieved by recombinant E. coli strains remain very low. Thus, Corynebacterium glutamicum, a highly efficient l-lysine producing microorganism, should be useful in the development of direct fermentative production of 5AVA using l-lysine as a precursor for 5AVA. Here, we report the development of metabolically engineered C. glutamicum strains for enhanced fermentative production of 5AVA from glucose. Results: Various expression vectors containing different promoters and origins of replication were examined for optimal expression of Pseudomonas putida davB and davA genes encoding lysine 2-monooxygenase and delta-aminovaleramidase, respectively. Among them, expression of the C. glutamicum codon-optimized davA gene fused with His 6 -Tag at its N-Terminal and the davB gene as an operon under a strong synthetic H 36 promoter (plasmid p36davAB3) in C. glutamicum enabled the most efficient production of 5AVA. Flask culture and fed-batch culture of this strain produced 6.9 and 19.7 g/L (together with 11.9 g/L glutaric acid as major byproduct) of 5AVA, respectively. Homology modeling suggested that endogenous gamma-aminobutyrate aminotransferase encoded by the gabT gene might be responsible for the conversion of 5AVA to glutaric acid in recombinant C. glutamicum. Fed-batch culture of a C. glutamicum gabT mutant-harboring p36davAB3 produced 33.1 g/L 5AVA with much reduced (2.0 g/L) production of glutaric acid. Conclusions:Corynebacterium glutamicum was successfully engineered to produce 5AVA from glucose by optimizing the expression of two key enzymes, lysine 2-monooxygenase and delta-aminovaleramidase. In addition, production of glutaric acid, a major byproduct, was significantly reduced by employing C. glutamicum gabT mutant as a host strain. The metabolically engineered C. glutamicum strains developed in this study should be useful for enhanced fermentative production of the novel C5 platform chemical 5AVA from renewable resources.

Författare

Jae Ho Shin

Korea Advanced Institute of Science and Technology (KAIST)

S Park

Korea Advanced Institute of Science and Technology (KAIST)

Y Oh

Korea Research Institute of Chemical Technology (KRICT)

J Choi

Korea Advanced Institute of Science and Technology (KAIST)

M Lee

Korea Advanced Institute of Science and Technology (KAIST)

J Cho

Korea Advanced Institute of Science and Technology (KAIST)

K Jeong

Korea Advanced Institute of Science and Technology (KAIST)

J Joo

Korea Research Institute of Chemical Technology (KRICT)

James Yu

Korea Advanced Institute of Science and Technology (KAIST)

S Park

Myongji University

S Lee

Korea Advanced Institute of Science and Technology (KAIST)

Microbial Cell Factories

1475-2859 (ISSN)

Vol. 15 1 174

Ämneskategorier

Biokemi och molekylärbiologi

Biomaterial

Bioprocessteknik

Mikrobiologi

Genetik

DOI

10.1186/s12934-016-0566-8

Mer information

Senast uppdaterat

2019-06-05