The global acetylome of the human pathogen vibrio cholerae V52 reveals lysine acetylation of major transcriptional regulators
Artikel i vetenskaplig tidskrift, 2018

Protein lysine acetylation is recognized as an important reversible post translational modification in all domains of life. While its primary roles appear to reside in metabolic processes, lysine acetylation has also been implicated in regulating pathogenesis in bacteria. Several global lysine acetylome analyses have been carried out in various bacteria, but thus far there have been no reports of lysine acetylation taking place in the important human pathogen Vibrio cholerae. In this study, we analyzed the lysine acetylproteome of the human pathogen V. cholerae V52. By applying a combination of immuno-enrichment of acetylated peptides and high resolution mass spectrometry, we identified 3,402 acetylation sites on 1,240 proteins. Of the acetylated proteins, more than half were acetylated on two or more sites. As reported for other bacteria, we observed that many of the acetylated proteins were involved in metabolic and cellular processes and there was an over-representation of acetylated proteins involved in protein synthesis. Of interest, we demonstrated that many global transcription factors such as CRP, H-NS, IHF, Lrp and RpoN as well as transcription factors AphB, TcpP, and PhoB involved in direct regulation of virulence in V. cholerae were acetylated. In conclusion, this is the first global protein lysine acetylome analysis of V. cholerae and should constitute a valuable resource for in-depth studies of the impact of lysine acetylation in pathogenesis and other cellular processes.

Författare

C. Jers

Danmarks Tekniske Universitet (DTU)

V. Ravikumar

Danmarks Tekniske Universitet (DTU)

Mateusz Lezyk

Danmarks Tekniske Universitet (DTU)

Abida Sultan

Danmarks Tekniske Universitet (DTU)

Åsa Sjöling

Karolinska universitetssjukhuset

Sun N. Wai

Umeå universitet

Ivan Mijakovic

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Frontiers in cellular and infection microbiology

2235-2988 (ISSN)

Vol. 7 JAN 537

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.3389/fcimb.2017.00537

Mer information

Senast uppdaterat

2018-05-29