Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout
Artikel i vetenskaplig tidskrift, 2018

A bioassay based on a high-Tc superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μl. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 e5 RCA coils (0.2e−18 mol), which is equivalent to 66 fM in the 3 μl sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.

POC

microfluidics

Bioassay

high-Tc SQUID

superconductivity

Författare

Sobhan Sepehri

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Emil Eriksson

Chalmers, Mikroteknologi och nanovetenskap (MC2)

Alexei Kalaboukhov

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Teresa Zardán Gómez de la Torre

Uppsala universitet

Kiryl Kustanovich

Chalmers, Kemi och kemiteknik, Kemi och biokemi, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi och kemiteknik, Kemi och biokemi

Justin Schneiderman

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Jakob Blomgren

Organisation okänd

Christer Johansson

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

Maria Strømme

Organisation okänd

Dag Winkler

Chalmers, Mikroteknologi och nanovetenskap (MC2), Kvantkomponentfysik

APL Bioengineering

2473-2877 (ISSN)

Vol. 2 1 016102

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Biokemi och molekylärbiologi

Analytisk kemi

Medicinsk bioteknik

Nanoteknik

Nanoteknik

Annan industriell bioteknik

Fundament

Grundläggande vetenskaper

Infrastruktur

Nanotekniklaboratoriet

DOI

10.1063/1.4999713

Mer information

Skapat

2018-02-08