Altered peripheral amino acid profile indicate a systemic impact of active celiac disease and a possible role of amino acids in disease pathogenesis
Artikel i vetenskaplig tidskrift, 2018

Background: We have previously performed a Genome Wide Association and linkage study that indicated a new disease triggering mechanism involving amino acid metabolism and nutrient sensing signaling pathways. Objective: The aim of this study was to investigate if plasma amino acid levels differed among children with celiac disease compared with disease controls. Materials and methods: Fasting plasma samples from 141 children with celiac disease and 129 non-celiac disease controls, were analyzed for amino acid levels by liquid chromatography-tandem mass spectrometry (LC/MS). A general linear model using age and experimental effects as covariates was used to compare amino acid levels between children with a diagnosis of celiac disease and controls. Results: Seven out of twenty-three analyzed amino acids were elevated in children with celiac disease compared with controls (tryptophan, taurine, glutamic acid, proline, ornithine, alanine and methionine). The significance of the individual amino acids do not survive multiple correction, however, multivariate analyses of the amino acid profile showed significantly altered amino acid levels in children with celiac disease overall and after correction for age, sex and experimental effects (p = 8.4 × 10 -8 ). Conclusion: These findings support the idea that amino acids could influence systemic inflammation and play a possible role in disease pathogenesis.

Författare

Åsa Torinsson Naluai

Göteborgs universitet

Ladan Saadat Vafa

Göteborgs universitet

Audur Gudjonsdottir

Sahlgrenska akademin

Henrik Arnell

Karolinska universitetssjukhuset

Lars Browaldh

Karolinska Institutet

Staffan Nilsson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Daniel Agardh

Lunds universitet

PLoS ONE

1932-6203 (ISSN)

Vol. 13 3 e0193764

Ämneskategorier

Pediatrik

Gastroenterologi

Näringslära

DOI

10.1371/journal.pone.0193764

Mer information

Senast uppdaterat

2018-03-23