Chronic coffee consumption in the diet-induced obese rat: impact on gut microbiota and serum metabolomics
Artikel i vetenskaplig tidskrift, 2014

Epidemiological data confirms a strong negative association between regular coffee consumption and the prevalence of type 2 diabetes. Coffee is initially absorbed in the stomach and small intestine but is further fermented in the colon by gut microbiota. The bioavailability, production and biological activity of coffee polyphenols is modulated, in part, by gut microbiota. The purpose of this study was to determine if chronic coffee consumption could mitigate negative gut microbiota and metabolomic profile changes induced by a high-fat diet. Male Sprague-Dawley rats were randomized to chow (12% kcal fat) or high-fat (60% kcal fat) diet. Each group was further divided into water or caffeinated coffee for 10 weeks. Coffee consumption in high-fat-fed rats was associated with decreased body weight, adiposity, liver triglycerides and energy intake. Despite a more favorable body composition, rats displayed profound systemic insulin resistance, likely due to caffeine. Coffee consumption attenuated the increase in Firmicutes (F)-to-Bacteroidetes (B) ratio and Clostridium Cluster XI normally associated with high-fat feeding but also resulted in augmented levels of Enterobacteria. In the serum metabolome, coffee had a distinct impact, increasing levels of aromatic and circulating short-chain fatty acids while lowering levels of branched-chain amino acids. In summary, coffee consumption is able to alter gut microbiota in high-fat-fed rats although the role of these changes in reducing diabetes risk is unclear given the increased insulin resistance observed with coffee in this study.

Gut microbiota

Metabolomics

Diet-induced obesity

Coffee

Författare

Theresa Cowan

University of Calgary

Marie Palmnäs

University of Calgary

Jane Yang

University of Calgary

Marc Bomhof

University of Calgary

Kendra Ardell

University of Calgary

Raylene Reimer

University of Calgary

Hans Vogel

University of Calgary

Jane Shearer

University of Calgary

Journal of Nutritional Biochemistry

0955-2863 (ISSN)

Vol. 25 489-95

Ämneskategorier

Cell- och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Näringslära

DOI

10.1016/j.jnutbio.2013.12.009