A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy
Artikel i vetenskaplig tidskrift, 2016

Cells can enter into a dormant state when faced with unfavorable conditions. However, how cells enter into and recover from this state is still poorly understood. Here, we study dormancy in different eukaryotic organisms and find it to be associated with a significant decrease in the mobility of organelles and foreign tracer particles. We show that this reduced mobility is caused by an influx of protons and a marked acidification of the cytoplasm, which leads to widespread macromolecular assembly of proteins and triggers a transition of the cytoplasm to a solid-like state with increased mechanical stability. We further demonstrate that this transition is required for cellular survival under conditions of starvation. Our findings have broad implications for understanding alternative physiological states, such as quiescence and dormancy, and create a new view of the cytoplasm as an adaptable fluid that can reversibly transition into a protective solid-like state.

Författare

Matthias Munder

Max-Planck-Gesellschaft

Daniel Midtvedt

Max-Planck-Gesellschaft

Titus Franzmann

Max-Planck-Gesellschaft

Elisabeth Nuske

Max-Planck-Gesellschaft

Oliver Otto

Technische Universität Dresden

Maik Herbig

Technische Universität Dresden

Elke Ulbricht

Technische Universität Dresden

Paul Müller

Technische Universität Dresden

Anna Taubenberger

Technische Universität Dresden

Shovamayee Maharana

Max-Planck-Gesellschaft

Liliana Malikovska

Max-Planck-Gesellschaft

Doris Richter

Max-Planck-Gesellschaft

Jochen Guck

Technische Universität Dresden

Vasily Zaburdaev

Max-Planck-Gesellschaft

Simon Alberti

Max-Planck-Gesellschaft

eLife

2050-084X (ISSN)

e09347

Ämneskategorier

Immunologi inom det medicinska området

Biofysik

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.7554/eLife.09347

Mer information

Senast uppdaterat

2019-08-22