Coinfection with enteric pathogens in east African children with acute gastroenteritis - Associations and interpretations
Artikel i vetenskaplig tidskrift, 2018

Enteric coinfections among children in low-income countries are very common, but it is not well known if specific pathogen combinations are associated or have clinical importance. In this analysis, feces samples from children in Rwanda and Zanzibar less than 5 years of age, with (N = 994) or without (N = 324) acute diarrhea, were analyzed by real-time polymerase chain reaction targeting a wide range of pathogens. Associations were investigated by comparing co-detection and mono-detection frequencies for all pairwise pathogen combinations. More than one pathogen was detected in 840 samples (65%). A negative association (coinfections being less common than expected from probability) was observed for rotavirus in combination with Shigella, Campylobacter, or norovirus genogroup II, but only in patients, which is statistically expected for agents that independently cause diarrhea. A positive correlation was observed, in both patients and controls, between Ct (threshold cycle) values for certain virulence factor genes in enteropathogenic Escherichia coli (EPEC) (eae and bfpA) and toxin genes in enterotoxigenic E. coli (eltB and estA), allowing estimation of how often these genes were present in the same bacteria. A significant positive association in patients only was observed for Shigella and EPEC-eae, suggesting that this coinfection might interact in a manner that enhances symptoms. Although interaction between pathogens that affect symptoms is rare, this work emphasizes the importance and difference in interpretation of coinfections depending on whether they are positively or negatively associated.

Författare

Maria Andersson

Göteborgs universitet

Jean-Claude Kabayiza

University of Rwanda

Kristina Elfving

Göteborgs universitet

Staffan Nilsson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Mwinyi I. Msellem

Ministry of Health and Social Welfare

Andreas Mårtensson

Uppsala universitet

Anders Björkman

Karolinska universitetssjukhuset

Tomas Bergström

Göteborgs universitet

Magnus Lindh

Göteborgs universitet

American Journal of Tropical Medicine and Hygiene

0002-9637 (ISSN)

Vol. 98 6 1566-1570

Ämneskategorier

Infektionsmedicin

Klinisk laboratoriemedicin

Medicinsk genetik

DOI

10.4269/ajtmh.17-0473

Mer information

Senast uppdaterat

2018-09-25