Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition
Artikel i vetenskaplig tidskrift, 2018

Control of molecular translocation through nanoscale apertures is of great interest for DNA sequencing, biomolecular filters, and new platforms for single molecule analysis. However, methods for controlling the permeability of nanopores are very limited. Here, we show how nanopores functionalized with poly(ethylene glycol) brushes, which fully prevent protein translocation, can be reversibly gated to an "open" state by binding of single IgG antibodies that disrupt the macromolecular barrier. On the basis of surface plasmon resonance data we propose a two-state model describing the antibody-polymer interaction kinetics. Reversibly (weakly) bound antibodies decrease the protein exclusion height while irreversibly (strongly) bound antibodies do not. Our results are further supported by fluorescence readout from pore arrays and high-speed atomic force microscopy on single pores. This type of dynamic barrier control on the nanoscale provides new possibilities for biomolecular separation and analysis.


Gustav Emilsson

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Yusuke Sakiyama

Universität Basel

Bita Malekian

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Kunli Xiong

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Zeynep Kaya

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Roderick Y H Lim

Universität Basel

Andreas Dahlin

Chalmers, Kemi och kemiteknik, Tillämpad kemi

ACS Central Science

23747943 (ISSN) 23747951 (eISSN)

Vol. 4 8 1007-1014


Biokemi och molekylärbiologi


Annan industriell bioteknik



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