Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development
Artikel i vetenskaplig tidskrift, 2018

Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different oligodendrocyte (OL) states at adult stages. Here, we present bulk and single-cell transcriptomics resources providing insights on how transitions between these states occur. We found that post-natal OPCs from brain and spinal cord present similar transcriptional signatures. Moreover, post-natal OPC progeny of E13.5 Pdgfra+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ pre-OPCs rewire their transcriptional network during development. Single-cell RNA-seq and lineage tracing indicates that a subset of E13.5 Pdgfra+ cells originates cells of the pericyte lineage. Thus, our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.

transcription factors

single-cell RNA-seq

platelet-derived growth factor receptor alpha

oligodendrocyte precursor cell

myelin

neural progenitors

transcriptomics

neural development

pericyte

oligodendrocyte

Författare

Sueli Marques

David van Bruggen

Darya Pavlovna Vanichkina

Elisa Mariagrazia Floriddia

Hermany Munguba

Stefania Giacomello

Ana Mendanha Falcão

Mandy Meijer

Åsa Kristina Björklund

Jens Hjerling-Leffler

Ryan James Taft

Gonçalo Castelo-Branco

Developmental cell

1878-1551 (eISSN)

Vol. 46 4 504-517

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Utvecklingsbiologi

DOI

10.1016/j.devcel.2018.07.005

PubMed

30078729

Mer information

Senast uppdaterat

2021-08-11