Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development
Artikel i vetenskaplig tidskrift, 2018

Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different oligodendrocyte (OL) states at adult stages. Here, we present bulk and single-cell transcriptomics resources providing insights on how transitions between these states occur. We found that post-natal OPCs from brain and spinal cord present similar transcriptional signatures. Moreover, post-natal OPC progeny of E13.5 Pdgfra+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ pre-OPCs rewire their transcriptional network during development. Single-cell RNA-seq and lineage tracing indicates that a subset of E13.5 Pdgfra+ cells originates cells of the pericyte lineage. Thus, our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.

oligodendrocyte

platelet-derived growth factor receptor alpha

myelin

transcriptomics

single-cell RNA-seq

neural development

pericyte

transcription factors

neural progenitors

oligodendrocyte precursor cell

Författare

Sueli Marques

Karolinska Institutet

David van Bruggen

Karolinska Institutet

Darya Pavlovna Vanichkina

The University of Sydney

Elisa Mariagrazia Floriddia

Karolinska Institutet

Hermany Munguba

Karolinska Institutet

Leif Väremo

Chalmers, Biologi och bioteknik

Stefania Giacomello

Stockholms universitet

Ana Mendanha Falcão

Karolinska Institutet

Mandy Meijer

Karolinska Institutet

Åsa Kristina Björklund

Uppsala universitet

Jens Hjerling-Leffler

Karolinska Institutet

Ryan James Taft

University of Queensland

Gonçalo Castelo-Branco

Karolinska Institutet

Developmental cell

1878-1551 (eISSN)

Vol. 46 4 504-517

Ämneskategorier

Cellbiologi

Cell- och molekylärbiologi

Utvecklingsbiologi

DOI

10.1016/j.devcel.2018.07.005

PubMed

30078729

Mer information

Senast uppdaterat

2018-09-03