3D Reconstruction of Porous and Poorly Conductive Soft Materials using FIB-SEM Tomography
The aim of this work was to develop a general protocol for optimisation of FIB-SEM tomography parameters for porous and poorly conductive soft materials.The optimised parameters include the energies and currents of the ion and electron beams, reduction of shadowing-effects, choice of electron detector and selection of method for charge neutralisation. In addition, a new self-learning binarisation algorithm is introduced to enable an automatic separation between pores and matrix. The binary data have been used to visualise the interconnectivity in 3D of individual pore paths through phase separated polymer films. The optimised protocol for FIB-SEM tomography is applicable to a variety of porous and poorly conductive soft materials.
The porous and poorly conductive soft materials in these studies were leached phase separated polymer films intended for controlled drug release coatings in pharmaceuticals. The porous microstructure within the films acts as transport path for the drug. In this work, the complex microstructure has been visualised in 3D. In addition, 3D visualisation of the shortest, intermediate and longest paths through the films, based upon tortuosity calculations, have been performed as well.
focused ion beam
scanning electron microscopy
Chalmers, Fysik, Eva Olsson Group
Soft Materials and Coatings for Controlled Drug Release Nanotechnologies in Preventive and Regenerative Medicine an Emerging Big Picture. V. Uskokovic and D. P. Uskokovic C. Fager and E. Olsson Netherlands: Elsevier. 2018, 244-259.
3D Reconstruction of Microstructure Using Optimised FIB-SEM Tomography Parameters for Porous and Poorly Conductive Soft Materials. C. Fager, M. Röding, A. Olsson, C. von Corswant, N. Lorén, A. Särkkä and E. Olsson Manuscript intended for Microscopy and Microanalysis
3D Visualisation of Individual Transport Paths in Controlled Drug Release Films using FIB-SEM Tomography C. Fager, S. E. Barman, M. Röding, A. Olsson, C. von Corswant, N. Lorén, A. Särkkä, H. Rotzén and E. Olsson Manuscript intended for International Journal of Pharmaceutics
Textil-, gummi- och polymermaterial
Chalmers tekniska högskola
Opponent: Dr. Anders Palmquist