Impact of forced fatty acid synthesis on metabolism and physiology of Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2018

Nutrient sensing and signaling controls the cellular response to extracellular nutrients and intracellular metabolites. Nutrient-dependent regulation of metabolism ensures balanced energy production and expenditure. We show that disturbing energy balance by forcing fatty acid synthesis has profound impact on metabolism and physiology of the yeast cell. In addition to an expected increase in storage lipids, we observed increased β-oxidation and reduced amino acid biosynthesis, indicating increased activity of nutrient-sensitive kinase Snf1p. We also observed increased sensitivity to rapamycin as well as decreased ribosome biogenesis and translation, indicating reduced activity of nutrient-sensitive kinase target of rapamycin complex 1. Additionally, we detected increased levels of oxidative stress and lower levels of amino acids. This study provides detailed insight into cellular resource redistribution in response to forced fatty acid synthesis and enables optimized engineering of microbial lipid production.

amino acid metabolism

Snf1p/AMPK

cell wall integrity signaling pathway

reactive oxygen species

lipid metabolism

TORC1

Författare

Michael Gossing

Chalmers, Biologi och bioteknik, Systembiologi

Agata Smialowska

Chalmers, Biologi och bioteknik, Systembiologi

National Bioinformatics Infrastructure Sweden (NBIS)

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Danmarks Tekniske Universitet (DTU)

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 18 8 foy096

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1093/femsyr/foy096

PubMed

30169781

Mer information

Senast uppdaterat

2019-03-04