Cervical microbiota in women with cervical intra-epithelial neoplasia, prior to and after local excisional treatment, a Norwegian cohort study
Artikel i vetenskaplig tidskrift, 2019

Background: Local treatment for cervical intraepithelial neoplasia (CIN) by Loop Electrosurgical Excision Procedure (LEEP) has been correlated with reproductive morbidity, while the cervicovaginal microbiota is also known to affect the risk of preterm delivery. CIN and treatment by LEEP might change the cervical microbiota. The main aim of this study was to describe the cervical microbiota before and after LEEP and assess its associaton with cone depth and HPV persistence. Further, we aimed to compare the microbiota to references with normal cervical cytology. Methods: Between 2005 and 2007, we prospectively identified 89 women planned for LEEP in a Norwegian hospital and recruited 100 references with a normal cervical cytology. Endocervical swabs were collected prior to treatment and at six (n = 77) and 12 months (n = 72) post LEEP for bacterial culture and PCR, and post LEEP for DNA testing for human papillomavirus (HPV). We compared the cervical microbiota composition before and after treatment and between women planned for LEEP vs references. Results: There was a reduction in the number of non-Lactobacillus bacterial species six and 12 months after LEEP compared to before treatment and a tendency towards a concomitant increase in Lactobacillus. No association between the detection of cervical bacteria, HPV persistence or cone depth was found. Women planned for LEEP carried significantly more Bacteroides spp., Gardnerella vaginalis, Mycoplasma hominis and Ureaplasma parvum as well as a greater number of bacterial species than the references. Conclusions: Local excisional treatment appears to alter the cervical microbiota towards a less diverse microbiota. Women with CIN have a more diverse cervical microbiota compared to women with normal cervical cytology.

Lactobacillus

CIN

Human papillomavirus

HPV

Cervical Intraepithelial Neoplasia

LEEP

Vaginal microbiota

Författare

Johanna Wiik

Sahlgrenska akademin

Østfold Hospital Trust

Verena Sengpiel

Sahlgrenska universitetssjukhuset

Maria Kyrgiou

Imperial College London

Imperial College Healthcare NHS Trust

Staffan Nilsson

Göteborgs universitet

Anita Mitra

Imperial College Healthcare NHS Trust

Imperial College London

Tom Tanbo

Oslo universitetssykehus

Christine Monceyron Jonassen

Østfold Hospital Trust

Tone Møller Tannæs

Akershus University Hospital

Katrine Sjøborg

Østfold Hospital Trust

BMC Womens Health

1472-6874 (ISSN)

Vol. 19 1 30

Ämneskategorier

Mikrobiologi inom det medicinska området

Klinisk vetenskap

Reproduktionsmedicin och gynekologi

DOI

10.1186/s12905-019-0727-0

PubMed

30728029

Mer information

Senast uppdaterat

2019-03-04