Regulatory Mechanisms of the Mucin-Like Region on Herpes Simplex Virus during Cellular Attachment
Artikel i vetenskaplig tidskrift, 2019

Mucin-like regions, characterized by a local high density of O-linked glycosylation, are found on the viral envelope glycoproteins of many viruses. Herpes simplex virus type 1 (HSV-1), for example, exhibits a mucin-like region on its glycoprotein gC, a viral protein involved in initial recruitment of the virus to the cell surface via interaction with sulfated glycosaminoglycans. So far, this mucin-like region has been proposed to play a key role in modulating the interactions with cellular glycosaminoglycans, and in particular to promote release of HSV-1 virions from infected cells. However, the molecular mechanisms and the role as a pathogenicity factor remains unclear. Using single virus particle tracking, we show that the mobility of chondroitin sulfate-bound HSV-1 virions is decreased in absence of the mucin-like region. This decrease in mobility correlates with an increase in HSV-1-chondroitin sulfate binding forces as observed using atomic force microscopy-based force spectroscopy. Our data suggest that the mucin-like region modulates virus-glycosaminoglycan interactions by regulating the affinity, type, and number of glycoproteins involved in the virus-glycosaminoglycan interaction. This study therefore presents new evidence for a role of the mucin-like region in balancing the interaction of HSV-1 with glycosaminoglycans and provides further insights into the molecular mechanisms used by the virus to ensure both successful cell entry and release from the infected cell.


Martin Delguste

Universite catholique de Louvain

Nadia Peerboom

Chalmers, Fysik, Biologisk fysik

Grégoire Le Brun

Universite catholique de Louvain

Edward Trybala

Göteborgs universitet

Sigvard Olofsson

Göteborgs universitet

Tomas Bergström

Göteborgs universitet

David Alsteens

Universite catholique de Louvain

Marta Bally

Umeå universitet

ACS Chemical Biology

1554-8929 (ISSN) 1554-8937 (eISSN)

Vol. 14 3 534-542



Cell- och molekylärbiologi

Mikrobiologi inom det medicinska området





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