Stiffness measurement of nanosized liposomes using solid-state nanopore sensor with automated recapturing platform
Artikel i vetenskaplig tidskrift, 2019

This paper describes a method to gauge the stiffness of nanosized liposomes – a nanoscale vesicle – using a custom-made recapture platform coupled to a solid-state nanopore sensor. The recapture platform electrically profiles a given liposome vesicle multiple times through automated reversal of the voltage polarity immediately following a translocation instance to re-translocate the same analyte through the nanopore – provides better statistical insight at the molecular level by analyzing the same particle multiple times compared to conventional nanopore platforms. The capture frequency depends on the applied voltage with lower voltages (i.e., 100 mV) permitting higher recapture instances than at higher voltages (>200 mV) since the probability of particles exiting the nanopore capture radius increases with voltage. The shape deformation was inferred by comparing the normalized relative current blockade ((Formula presented.) at the two voltage polarities to that of a rigid particle, i.e., polystyrene beads. We found that liposomes deform to adopt a prolate shape at higher voltages. This platform can be further applied to investigate the stiffness of other types of soft matters, e.g., virus, exosomes, endosomes, and accelerate the potential studies in pharmaceutics for increasing the drug packing and unpacking mechanism by controlling the stiffness of the drug vesicles.

Nanoparticle

Recapturing platform

Solid-state nanopore

Stiffness

Liposome

Författare

Jung Soo Lee

Southern Methodist University

Jugal Saharia

Southern Methodist University

Y. M.Nuwan D.Y. Bandara

Southern Methodist University

Buddini Iroshika Karawdeniya

Southern Methodist University

Gaurav Goyal

Chalmers, Biologi och bioteknik

Armin Darvish

Quantapore Inc.

Qingxiao Wang

University of Texas at Dallas

Moon J. Kim

University of Texas at Dallas

Min Jun Kim

Southern Methodist University

Electrophoresis

0173-0835 (ISSN) 1522-2683 (eISSN)

Vol. 40 9 1337-1344

Ämneskategorier

Annan kemiteknik

Annan elektroteknik och elektronik

Den kondenserade materiens fysik

DOI

10.1002/elps.201800476

Mer information

Senast uppdaterat

2019-07-26