Single-molecule fluorescence studies of intrinsically disordered proteins and liquid phase separation
Reviewartikel, 2019

Intrinsically disordered proteins (IDPs) are ubiquitous in proteomes and serve in a range of cellular functions including signaling, regulation, transport and enzyme function. IDP misfunction and aggregation are also associated with several diseases including neurodegenerative diseases and cancer. During the past decade, single-molecule methods have become popular for detailed biophysical and structural studies of these complex proteins. This work has included recent applications to cellular liquid-liquid phase separation (LLPS), relevant for functional dynamics of membraneless organelles such as the nucleolus and stress granules. In this concise review, we cover the conceptual motivations for development and application of single-molecule fluorescence methods for such IDP studies. We follow with a few key examples of systems and biophysical problems that have been addressed, and conclude with thoughts for emerging and future directions.

Liquid-liquid phase separation

Single-molecule biophysics

Single-molecule FRET

Intrinsically disordered proteins

Författare

Irem Nasir

Scripps Research Institute

Chalmers, Biologi och bioteknik, Kemisk biologi

Paulo L. Onuchic

Scripps Research Institute

Sergio R. Labra

Scripps Research Institute

Ashok A. Deniz

Scripps Research Institute

Biochimica et Biophysica Acta - Proteins and Proteomics

1570-9639 (ISSN)

Vol. 1867 10 980-987

Ämneskategorier

Biokemi och molekylärbiologi

Biofysik

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1016/j.bbapap.2019.04.007

PubMed

31054969

Mer information

Senast uppdaterat

2019-11-19