Quantitative Mapping of Triacylglycerol Chain Length and Saturation Using Broadband CARS Microscopy
Artikel i vetenskaplig tidskrift, 2019

Lipid droplets (LDs), present in many cell types, are highly dynamic organelles that store neutral lipids, primarily triacylglycerols (TAGs). With the discovery of new LD functions (e.g., in immune response, protein clearage, and occurrence with disease), new methods to study LD chemical composition in situ are necessary. We present an approach for in situ, quantitative TAG analysis using label-free, coherent Raman microscopy that allows deciphering LD TAG composition in different biochemically complex samples with submicrometer spatial resolution. Employing a set of standard TAGs, we generate a spectral training matrix capturing the variation caused in Raman-like spectra by TAG backbone, chain length, and number of double bonds per chain, as well as the presence of proteins or other diluting molecules. Comparing our fitting approach to gas chromatography measurements for mixtures of standard TAGs and food oils, we find the root mean-square error for the prediction of TAG chemistry to be 0.69 CH2 and 0.15 #C=C. When progressing to more complex samples such as oil emulsions and LDs in various eukaryotic cells, we find good agreement with bulk gas chromatography measurements. For differentiated adipocytes, we find a significant increase in the number of double bonds in small LDs (below 2 μm in diameter) compared to large LDs (above 2 μm in diameter). Coupled with a relatively limited sample preparation requirement, this approach should enable rapid and accurate TAG LD analysis for a variety of cell biology and technological applications.

lipids

microscopy

Raman

Författare

Yujen Wang

Max Planck Institutet för Polymerforskning

Alexandra Paul

Chalmers, Biologi och bioteknik, Kemisk biologi

Cecilia Brännmark

Göteborgs Universitet

Sachin Kumar

Max Planck Institutet för Polymerforskning

Mischa Bonn

Max Planck Institutet för Polymerforskning

Sapun H. Parekh

The University of Texas at Austin

Biophysical Journal

0006-3495 (ISSN) 1542-0086 (eISSN)

Female Investigators in Nonlinear Optical Nanoscopy - FINON (FINON)

Europeiska kommissionen (FP7), 2013-10-01 -- 2017-09-30.

Ämneskategorier

Fysiologi

Annan fysik

Biofysik

DOI

10.1016/j.bpj.2019.04.036

Mer information

Skapat

2019-06-10