Hybrid vesicles as intracellular reactive oxygen species and nitric oxide generators
Artikel i vetenskaplig tidskrift, 2019

Artificial organelles are envisioned as nanosized assemblies with intracellular biocatalytic activity to provide the host cells with non-native or missing/lost function. Hybrid vesicles loaded with glucose oxidase (NRGOx) or beta-galactosidase (NR beta-Gal) and equipped with lysosomal escape ability are assembled using phospholipids and the block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). The co-localization of the building blocks and the catalytic activity of NRGOx and NR beta-Gal are illustrated. The intracellular activity of the nanoreactors in RAW 264.7 macrophages is confirmed by an enhanced reduction in viability for cells pre-incubated with NRGOx in the presence of glucose due to the generation of cytotoxic hydrogen peroxide compared to the controls. In addition, RAW 264.7 macrophages and primary human macrophages equipped with NR beta-Gal are able to intracellularly convert beta-Gal-NONOate into nitric oxide. The successful use of these hybrid vesicles to equip host macrophages with additional catalytic activity diversifies the available toolbox of nanocarriers with envisioned application in cell mimicry.

Författare

Yan Zhang

Aarhus Universitet

Noga Gal

Aarhus Universitet

Fabian Itel

Aarhus Universitet

Isabella N. Westensee

Aarhus Universitet

Edit Brodszkij

Aarhus Universitet

Daniel Mayer

Universitätsklinikum Ulm

Steffen Stenger

Universitätsklinikum Ulm

Miquel Castellote-Borrell

Aarhus Universitet

Thomas Boesen

Aarhus Universitet

Seyed Tabaei

Chalmers, Fysik, Biologisk fysik

Fredrik Höök

Chalmers, Fysik, Biologisk fysik

Brigitte Stadler

Aarhus Universitet

Nanoscale

2040-3364 (ISSN)

Vol. 11 24 11530-11541

Ämneskategorier

Cellbiologi

Immunologi

Cell- och molekylärbiologi

DOI

10.1039/c9nr02584g

PubMed

31150038

Mer information

Senast uppdaterat

2019-12-16