Industrial wastewater treatment plant enriches antibiotic resistance genes and alters the structure of microbial communities
Artikel i vetenskaplig tidskrift, 2019

Antibiotic resistance is an emerging global health crisis, driven largely by overuse and misuse of antibiotics. However, there are examples in which the production of these antimicrobial agents has polluted the environment with active antibiotic residues, selecting for antibiotic resistant bacteria and the genes they carry. In this work, we have used shotgun metagenomics to investigate the taxonomic structure and resistance gene composition of sludge communities in a treatment plant in Croatia receiving wastewater from production of the macrolide antibiotic azithromycin. We found that the total abundance of antibiotic resistance genes was three times higher in sludge from the treatment plant receiving wastewater from pharmaceutical production than in municipal sludge from a sewage treatment plant in Zagreb. Surprisingly, macrolide resistance genes did not have higher abundances in the industrial sludge, but genes associated with mobile genetic elements such as integrons had. We conclude that at high concentrations of antibiotics, selection may favor taxonomic shifts towards intrinsically resistant species or strains harboring chromosomal resistance mutations rather than acquisition of mobile resistance determinants. Our results underscore the need for regulatory action also within Europe to avoid release of antibiotics into the environment.

Antibiotic resistance

Pharmaceutical production

Wastewater treatment

Community structure

Macrolides

Författare

Johan Bengtsson-Palme

Göteborgs universitet

Center for Antibiotic Resistance Research

University of Wisconsin Madison

Milena Milakovic

Ruder Boskovic Institute

Helena Švecová

Jihoceska Univerzita v Ceskuch Budejovicich

Marin Ganjto

Zagreb

Viktor Jonsson

CSBI

R. Grabic

Jihoceska Univerzita v Ceskuch Budejovicich

Nikolina Udikovic-Kolic

Ruder Boskovic Institute

Water Research

0043-1354 (ISSN)

Vol. 162 437-445

Ämneskategorier

Botanik

Mikrobiologi

Genetik och förädling

DOI

10.1016/j.watres.2019.06.073

Mer information

Senast uppdaterat

2019-10-14