The serial millisecond crystallography instrument at the Australian Synchrotron incorporating the "lipidico" injector
Artikel i vetenskaplig tidskrift, 2019

A serial millisecond crystallography (SMX) facility has recently been implemented at the macromolecular crystallography beamline, MX2 at the Australian Synchrotron. The setup utilizes a combination of an EIGER X 16M detector system and an in-house developed high-viscosity injector, "Lipidico." Lipidico uses a syringe needle to extrude the microcrystal-containing viscous media and it is compatible with commercially available syringes. The combination of sample delivery via protein crystals suspended in a viscous mixture and a millisecond frame rate detector enables high-Throughput serial crystallography at the Australian Synchrotron. A hit-finding algorithm, based on the principles of "robust-statistics," is employed to rapidly process the data. Here we present the first SMX experimental results with a detector frame rate of 100 Hz (10 ms exposures) and the Lipidico injector using a mixture of lysozyme microcrystals embedded in high vacuum silicon grease. Details of the experimental setup, sample injector, and data analysis pipeline are designed and developed as part of the Australian Synchrotron SMX instrument and are reviewed here.

Författare

Peter Berntsen

La Trobe University

M. Hadian Jazi

La Trobe University

M. Kusel

Kusel Design

A. V. Martin

RMIT University

Thomas Ericsson

Matematik

M. J. Call

Walter and Eliza Hall Institute of Medical Research

University of Melbourne

R. Trenker

Walter and Eliza Hall Institute of Medical Research

University of Melbourne

F. G. Roque

La Trobe University

Connie Darmanin

La Trobe University

Brian Abbey

La Trobe University

Review of Scientific Instruments

0034-6748 (ISSN)

Vol. 90 8 085110

Ämneskategorier

Acceleratorfysik och instrumentering

Bioinformatik (beräkningsbiologi)

Bioinformatik och systembiologi

DOI

10.1063/1.5104298

PubMed

31472610

Mer information

Senast uppdaterat

2019-11-08