Homogeneous differential magnetic assay
Artikel i vetenskaplig tidskrift, 2019
Assays are widely used for detection of various targets, including pathogens, drugs, and toxins. Homogeneous assays are promising for the realization of point-of-care diagnostics as they do not require separation, immobilization, or washing steps. For low concentrations of target molecules, the speed and sensitivity of homogeneous assays have hitherto been limited by slow binding kinetics, time-consuming amplification steps, and the presence of a high background signal. Here, we present a homogeneous differential magnetic assay that utilizes a differential magnetic readout that eliminates previous limitations of homogeneous assays. The assay uses a gradiometer sensor configuration combined with precise microfluidic sample handling. This enables simultaneous differential measurement of a positive test sample containing a synthesized Vibrio cholerae target and a negative control sample, which reduces the background signal and increases the readout speed. Very low concentrations of targets down to femtomolar levels are thus detectable without any additional amplification of the number of targets. Our homogeneous differential magnetic assay method opens new possibilities for rapid and highly sensitive diagnostics at the point of care.
homogeneous differential magnetic assay
microfluidics
Brownian relaxation
magnetic nanoparticles
rolling circle amplification
Författare
Sobhan Sepehri
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Teresa Zardán Gómez De La Torre
Uppsala universitet
Justin Schneiderman
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Jakob Blomgren
RISE Research Institutes of Sweden
Aldo Jesorka
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Christer Johansson
Chalmers, Mikroteknologi och nanovetenskap
Mats Nilsson
Science for Life Laboratory (SciLifeLab)
Jan Albert
Karolinska universitetssjukhuset
Karolinska Institutet
Maria Strømme
Uppsala universitet
Dag Winkler
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Alexei Kalaboukhov
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
ACS Sensors
23793694 (eISSN)
Vol. 4 9 2381-2388Bärbar influensa diagnostic "FLU-ID"
Stiftelsen för Strategisk forskning (SSF) (SBE13-0125), 2021-01-01 -- 2021-03-31.
Stiftelsen för Strategisk forskning (SSF) (SBE13-0125), 2014-06-01 -- 2020-12-31.
Styrkeområden
Nanovetenskap och nanoteknik
Ämneskategorier
Analytisk kemi
Medicinsk apparatteknik
Annan fysik
Biomedicinsk laboratorievetenskap/teknologi
Nanoteknik
Annan industriell bioteknik
Drivkrafter
Innovation och entreprenörskap
Infrastruktur
Nanotekniklaboratoriet
DOI
10.1021/acssensors.9b00969