Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2015

Stationary-phase (SP) batch cultures of Saccharomyces cerevisiae, in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan, quiescence and SP-associated robustness. Based on this type of experiments, typically performed under aerobic conditions, several roles of oxygen in aging have been proposed. However, SP in anaerobic yeast cultures has not been investigated in detail. Here, we use the unique capability of S. cerevisiae to grow in the complete absence of oxygen to directly compare SP in aerobic and anaerobic bioreactor cultures. This comparison revealed strong positive effects of oxygen availability on adenylate energy charge, longevity and thermotolerance during SP. A low thermotolerance of anaerobic batch cultures was already evident during the exponential growth phase and, in contrast to the situation in aerobic cultures, was not substantially increased during transition into SP. A combination of physiological and transcriptome analysis showed that the slow post-diauxic growth phase on ethanol, which precedes SP in aerobic, but not in anaerobic cultures, endowed cells with the time and resources needed for inducing longevity and thermotolerance. When combined with literature data on acquisition of longevity and thermotolerance in retentostat cultures, the present study indicates that the fast transition from glucose excess to SP in anaerobic cultures precludes acquisition of longevity and thermotolerance. Moreover, this study demonstrates the importance of a preceding, calorie-restricted conditioning phase in the acquisition of longevity and stress tolerance in SP yeast cultures, irrespective of oxygen availability.

Anaerobiosis

Transcriptional response

Stationary phase

Energetics

Chronological lifespan

Thermotolerance

Conditioning

Författare

Mark Bisschops

TU Delft

Novo Nordisk Fonden

Chalmers, Biologi och bioteknik, Systembiologi

Tim Vos

TU Delft

Rubén Martínez-Moreno

Universidad de La Rioja

Quercus Europe s.l.

Pilar de la Torre Cortés

TU Delft

J. Pronk

TU Delft

P. Daran-Lapujade

TU Delft

Microbial Cell

23112638 (eISSN)

Vol. 2 11 429-444

Ämneskategorier

Atom- och molekylfysik och optik

Annan kemiteknik

Den kondenserade materiens fysik

DOI

10.15698/mic2015.11.238

Mer information

Senast uppdaterat

2019-11-14