Macromolecular nanocrystal structural analysis with electron and X-rays: A comparative review
Reviewartikel, 2019

Crystallography has long been the unrivaled method that can provide the atomistic structural models of macromolecules, using either X-rays or electrons as probes. The methodology has gone through several revolutionary periods, driven by the development of new sources, detectors, and other instrumentation. Novel sources of both X-ray and electrons are constantly emerging. The increase in brightness of these sources, complemented by the advanced detection techniques, has relaxed the traditionally strict need for large, high quality, crystals. Recent reports suggest high-quality diffraction datasets from crystals as small as a few hundreds of nanometers can be routinely obtained. This has resulted in the genesis of a new field of macromolecular nanocrystal crystallography. Here we will make a brief comparative review of this growing field focusing on the use of X-rays and electrons sources.

X-ray free-electron laser

Electron diffraction

Nanocrystallography

Författare

Krishna P. Khakurel

Czech Academy of Sciences

Borislav Angelov

Czech Academy of Sciences

Jakob Andreasson

Chalmers, Fysik, Kondenserade materiens fysik

Czech Academy of Sciences

Molecules

1420-3049 (ISSN)

Vol. 24 19 3490

Ämneskategorier

Acceleratorfysik och instrumentering

Oorganisk kemi

Astronomi, astrofysik och kosmologi

Styrkeområden

Materialvetenskap

DOI

10.3390/molecules24193490

PubMed

31561479

Mer information

Senast uppdaterat

2019-12-03