Self-Establishing Communities: A Yeast Model to Study the Physiological Impact of Metabolic Cooperation in Eukaryotic Cells
Artikel i vetenskaplig tidskrift, 2019

All biosynthetically active cells are able to export and import metabolites, the small molecule intermediaries of metabolism. In dense cell populations, this hallmark of cells results in the intercellular exchange of a wide spectrum of metabolites. Such metabolite exchange enables metabolic specialization of individual cells, leading to far reaching biological implications, as a consequence of the intrinsic connection between metabolism and cell physiology. In this chapter, we discuss methods on how to study metabolite exchange interactions by using self-establishing metabolically cooperating communities (SeMeCos) in the budding yeast Saccharomyces cerevisiae. SeMeCos exploit the stochastic segregation of episomes to progressively increase the number of essential metabolic interdependencies in a community that grows out from an initially prototrophic cell. By coupling genotype to metabotype, SeMeCos allow for the tracking of cells while they specialize metabolically and hence the opportunity to study their progressive change in physiology.

Metabolic specialization

Metabolic cooperation

Yeast communities


Kate Campbell

Chalmers, Biologi och bioteknik, Systembiologi

Clara Correia-Melo

University of Cambridge

The Francis Crick Institute

M. Ralser

Charité Universitätsmedizin Berlin

The Francis Crick Institute

Methods in molecular biology (Clifton, N.J.)

19406029 (eISSN)

Vol. 2049 263-282



Annan medicinsk bioteknologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)





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