Complex I is bypassed during high intensity exercise
Artikel i vetenskaplig tidskrift, 2019

Human muscles are tailored towards ATP synthesis. When exercising at high work rates muscles convert glucose to lactate, which is less nutrient efficient than respiration. There is hence a trade-off between endurance and power. Metabolic models have been developed to study how limited catalytic capacity of enzymes affects ATP synthesis. Here we integrate an enzyme-constrained metabolic model with proteomics data from muscle fibers. We find that ATP synthesis is constrained by several enzymes. A metabolic bypass of mitochondrial complex I is found to increase the ATP synthesis rate per gram of protein compared to full respiration. To test if this metabolic mode occurs in vivo, we conduct a high resolved incremental exercise tests for five subjects. Their gas exchange at different work rates is accurately reproduced by a whole-body metabolic model incorporating complex I bypass. The study therefore shows how proteome allocation influences metabolism during high intensity exercise.


Avlant Nilsson

Chalmers, Biologi och bioteknik, Systembiologi

Elias Björnson

Wallenberg Lab.

Chalmers, Biologi och bioteknik, Systembiologi

Mikael Flockhart

Gymnastik- och idrottshögskolan (GIH)

Filip J. Larsen

Gymnastik- och idrottshögskolan (GIH)

Jens B Nielsen

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Novo Nordisk Fonden

Nature Communications

2041-1723 (ISSN) 20411723 (eISSN)

Vol. 10 1 5072


Farmaceutisk vetenskap







Mer information

Senast uppdaterat