High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus
Artikel i vetenskaplig tidskrift, 2019

Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.

Wickerhamomyces anomalus

Cachaça

Glycerol metabolism

STL1

Biotechnological applications

Glycerol transport

Författare

Aureliano Claret da Cunha

Universidade Federal de Ouro Preto

Lorena Soares Gomes

Universidade Federal de Ouro Preto

Fernanda Godoy-Santos

Universidade Federal de Ouro Preto

Fábio Luis Da Silva Faria Oliveira

Universidade Federal de Ouro Preto

Janaína Aparecida Teixeira

Universidade Federal de Ouro Preto

Geraldo Magela Santos Sampaio

Universidade Federal de Ouro Preto

Maria José Magalhães Trópia

Universidade Federal de Ouro Preto

Ieso Miranda Castro Castro

Universidade Federal de Ouro Preto

Cândida Lucas

Universidade Federal de Ouro Preto

Rogelio Lopes Brandão

Universidade Federal de Ouro Preto

Journal of Industrial Microbiology and Biotechnology

1367-5435 (ISSN) 1476-5535 (eISSN)

Vol. 46 5 709-723

Ämneskategorier

Livsmedelsvetenskap

Biokatalys och enzymteknik

Annan industriell bioteknik

DOI

10.1007/s10295-018-02119-5

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Senast uppdaterat

2021-02-26