Manually curated genome-scale reconstruction of the metabolic network of Bacillus megaterium DSM319
Artikel i vetenskaplig tidskrift, 2019

Bacillus megaterium is a microorganism widely used in industrial biotechnology for production of enzymes and recombinant proteins, as well as in bioleaching processes. Precise understanding of its metabolism is essential for designing engineering strategies to further optimize B. megaterium for biotechnology applications. Here, we present a genome-scale metabolic model for B. megaterium DSM319, iJA1121, which is a result of a metabolic network reconciliation process. The model includes 1709 reactions, 1349 metabolites, and 1121 genes. Based on multiple-genome alignments and available genome-scale metabolic models for other Bacillus species, we constructed a draft network using an automated approach followed by manual curation. The refinements were performed using a gap-filling process. Constraint-based modeling was used to scrutinize network features. Phenotyping assays were performed in order to validate the growth behavior of the model using different substrates. To verify the model accuracy, experimental data reported in the literature (growth behavior patterns, metabolite production capabilities, metabolic flux analysis using C-13 glucose and formaldehyde inhibitory effect) were confronted with model predictions. This indicated a very good agreement between in silico results and experimental data. For example, our in silico study of fatty acid biosynthesis and lipid accumulation in B. megaterium highlighted the importance of adopting appropriate carbon sources for fermentation purposes. We conclude that the genome-scale metabolic model iJA1121 represents a useful tool for systems analysis and furthers our understanding of the metabolism of B. megaterium.

Författare

Javad Aminian-Dehkordi

Tarbiat Modares University

Seyyed Mohammad Mousavi

Tarbiat Modares University

Arezou Jafari

Tarbiat Modares University

Ivan Mijakovic

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Sayed-Amir Marashi

University of Tehran

Scientific Reports

2045-2322 (ISSN)

Vol. 9 18762

Ämneskategorier

Bioinformatik (beräkningsbiologi)

Bioinformatik och systembiologi

Datavetenskap (datalogi)

DOI

10.1038/s41598-019-55041-w

PubMed

31822710

Mer information

Senast uppdaterat

2020-01-28