Controlled Loading and Release of Beta-Lactoglobulin in Calcium-Polygalacturonate Hydrogels
Artikel i vetenskaplig tidskrift, 2020

We show here how the structure of polygalacturonate (polyGalA) hydrogels cross-linked by Ca2+ cations via external gelation controls the loading and release rate of beta-lactoglobulin (BLG), a globular protein. Hydrogels prepared from a polyGalA/BLG solution are found to be similar to those obtained from a polyGalA solution in our previous study (Maire du Poset et al. Biomacromolecules 2019, 20 (7), 2864-2872): they exhibit similar transparencies and gradients of mechanical properties and polyGalA concentrations. The nominal BLG/polyGalA ratio of the mixtures is almost recovered within the whole mixed hydrogel despite such strong concentration gradients, except in the part of the hydrogels with the largest mesh size, where more BLG proteins are present. This gradient enables one to tune the amount of protein loaded within the hydrogel. At a local scale, the proteins are distributed evenly within the hydrogel network, as shown by small-angle neutron scattering (SANS). The release of proteins from hydrogels is driven by Fickian diffusion, and the release rate increases with the mesh size of the network, with a characteristic time of a few hours. The specific structure of these polysaccharide-based hydrogels allows for control of both the dosage and the release rate of the loaded protein and makes them good candidates for use as oral controlled-delivery systems.


Aline Maire Du Poset

Université Bourgogne Franche-Comté

Université Paris-Saclay

SOLEIL Synchrotron

Mikaela Börjesson

Université Paris-Saclay

Chalmers, Kemi och kemiteknik, Tillämpad kemi

Céline Rameau

Université Bourgogne Franche-Comté

Claire Madeleine-Perdrillat

Université Paris-Saclay

Adrien Lerbret

Université Bourgogne Franche-Comté

Camille Loupiac

Université Paris-Saclay

Université Bourgogne Franche-Comté

Fabrice Cousin

Université Paris-Saclay

Ali Assifaoui

Université Bourgogne Franche-Comté


1525-7797 (ISSN) 1526-4602 (eISSN)

Vol. 21 4 1417-1426



Fysikalisk kemi






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