De Novo Biosynthesis of Caffeic Acid from Glucose by Engineered Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2020

Caffeic acid is a plant phenolic compound possessing extensive pharmacological activities. Here, we identified that p-coumaric acid 3-hydroxylase from Arabidopsis thaliana was capable of hydroxylating p-coumaric acid to form caffeic acid in Saccharomyces cerevisiae. Then, we introduced a combined caffeic acid biosynthetic pathway into S. cerevisiae and obtained 0.183 mg L-1 caffeic acid from glucose. Next we improved the tyrosine biosynthesis in S. cerevisiae by blocking the pathway flux to aromatic alcohols and eliminating the tyrosine-induced feedback inhibition resulting in caffeic acid production of 2.780 mg L-1. Finally, the medium was optimized, and the highest caffeic acid production obtained was 11.432 mg L-1 in YPD medium containing 4% glucose. This study opens a route to produce caffeic acid from glucose in S. cerevisiae and establishes a platform for the biosynthesis of caffeic acid derived metabolites.

Saccharomyces cerevisiae

caffeic acid

metabolic engineering

p-coumaric acid 3-hydroxylase

de novo


Yuanzi Li

Nankai University

Jiwei Mao

Chalmers, Biologi och bioteknik, Systembiologi

Quanli Liu

Chalmers, Biologi och bioteknik, Systembiologi

Xiaofei Song

Nankai University

Yuzhen Wu

Nankai University

Miao Cai

Nankai University

Haijin Xu

Nankai University

Mingqiang Qiao

Nankai University

ACS Synthetic Biology

2161-5063 (eISSN)

Vol. 9 4 756-765


Annan medicinsk grundvetenskap


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