A Sustainable Approach for the Green Synthesis of Silver Nanoparticles from Solibacillus isronensis sp. and Their Application in Biofilm Inhibition
Artikel i vetenskaplig tidskrift, 2020

The use of bacteria as nanofactories for the green synthesis of nanoparticles is considered a sustainable approach, owing to the stability, biocompatibility, high yields and facile synthesis of nanoparticles. The green synthesis provides the coating or capping of biomolecules on nanoparticles surface, which confer their biological activity. In this study, we report green synthesis of silver nanoparticles (AgNPs) by an environmental isolate; named as AgNPs1, which showed 100% 16S rRNA sequence similarity with Solibacillus isronensis. UV/visible analysis (UV/Vis), transmission electron microscopy (TEM), atomic force microscopy (AFM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR) were used to characterize the synthesized nanoparticles. The stable nature of nanoparticles was studied by thermogravimetric analysis (TGA) and inductively coupled plasma mass spectrometry (ICP-MS). Further, these nanoparticles were tested for biofilm inhibition against Escherichia coli and Pseudomonas aeruginosa. The AgNPs showed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 3.12 µg/mL and 6.25 µg/mL for E. coli, and 1.56 µg/mL and 3.12 µg/mL for P. aeruginosa, respectively.

silver nanoparticles (AgNPs), biofilm inhibition

E. coli

Solibacillus isronensis

P. aeruginosa

Författare

Priyanka Singh

Danmarks Tekniske Universitet (DTU)

Santosh Pandit

Chalmers, Biologi och bioteknik, Systembiologi

Venkata Raghavendra Subrahmanya Sar Mokkapati

Chalmers, Kemi och kemiteknik, Energi och material, Oorganisk miljökemi 2

Jørgen Garnæs

Dansk Fundamental Metrologi AS

Ivan Mijakovic

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi

Molecules

1420-3049 (ISSN)

Vol. 25 12

Ämneskategorier

Oorganisk kemi

Materialkemi

Annan kemi

DOI

10.3390/molecules25122783

PubMed

32560208

Mer information

Senast uppdaterat

2020-08-12