Large Changes in Protonation of Weak Polyelectrolyte Brushes with Salt Concentration-Implications for Protein Immobilization
Artikel i vetenskaplig tidskrift, 2020

We report for the first time that the protonation behavior of weak polyelectrolyte brushes depends very strongly on ionic strength. The pKa changes by one pH step per order of magnitude in salt concentration. For low salt concentrations (∼1 mM), a very high pH is required to deprotonate a polyacidic brush and a very low pH is required to protonate a polybasic brush. This has major consequences for interactions with other macromolecules, as the brushes are actually almost fully neutral when believed to be charged. We propose that many previous studies on electrostatic interactions between polyelectrolytes and proteins have, in fact, looked at other types of intermolecular forces, in particular, hydrophobic interactions and hydrogen bonds.

Författare

Gustav Ferrand-Drake Del Castillo

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Andreas Dahlin Group

Rebekah Hailes

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Andreas Dahlin Group

Andreas Dahlin

Chalmers, Kemi och kemiteknik, Tillämpad kemi, Andreas Dahlin Group

Journal of Physical Chemistry Letters

1948-7185 (eISSN)

Vol. 11 13 5212-5218

Ämneskategorier

Fysikalisk kemi

Biofysik

Teoretisk kemi

DOI

10.1021/acs.jpclett.0c01289

PubMed

32515599

Mer information

Senast uppdaterat

2020-08-17