Importance of protein Ser/Thr/Tyr phosphorylation for bacterial pathogenesis
Reviewartikel, 2020

Protein phosphorylation regulates a large variety of biological processes in all living cells. In pathogenic bacteria, the study of serine, threonine, and tyrosine (Ser/Thr/Tyr) phosphorylation has shed light on the course of infectious diseases, from adherence to host cells to pathogen virulence, replication, and persistence. Mass spectrometry (MS)-based phosphoproteomics has provided global maps of Ser/Thr/Tyr phosphosites in bacterial pathogens. Despite recent developments, a quantitative and dynamic view of phosphorylation events that occur during bacterial pathogenesis is currently lacking. Temporal, spatial, and subpopulation resolution of phosphorylation data is required to identify key regulatory nodes underlying bacterial pathogenesis. Herein, we discuss how technological improvements in sample handling, MS instrumentation, data processing, and machine learning should improve bacterial phosphoproteomic datasets and the information extracted from them. Such information is expected to significantly extend the current knowledge of Ser/Thr/Tyr phosphorylation in pathogenic bacteria and should ultimately contribute to the design of novel strategies to combat bacterial infections.

omics integration


Hanks kinases

antibiotic kinase inhibitors


antibiotic resistance

Tyr phosphorylation

machine learning

pathogenic bacteria


host-pathogen interactions



Julie Bonne Kohler

Danmarks Tekniske Universitet (DTU)

Carsten Jers

Danmarks Tekniske Universitet (DTU)

Meriem Senissar

Danmarks Tekniske Universitet (DTU)

Lei Shi

Chalmers, Biologi och bioteknik, Systembiologi

Abderahmane Derouiche

Chalmers, Biologi och bioteknik, Systembiologi

Ivan Mijakovic

Novo Nordisk Foundation Center for Biosustainability

Chalmers, Biologi och bioteknik, Systembiologi

FEBS Letters

0014-5793 (ISSN)

Vol. 594 15 2339-2369



Bioinformatik och systembiologi





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