Evolutionary Analysis of the Bacillus subtilis Genome Reveals New Genes Involved in Sporulation
Artikel i vetenskaplig tidskrift, 2020
Bacilli can form dormant, highly resistant, and metabolically inactive spores to cope with extreme environmental challenges. In this study, we examined the evolutionary age of Bacillus subtilis sporulation genes using the approach known as genomic phylostratigraphy. We found that B. subtilis sporulation genes cluster in several groups that emerged at distant evolutionary time-points, suggesting that the sporulation process underwent several stages of expansion. Next, we asked whether such evolutionary stratification of the genome could be used to predict involvement in sporulation of presently uncharacterized genes (y-genes). We individually inactivated a representative sample of uncharacterized genes that arose during the same evolutionary periods as the known sporulation genes and tested the resulting strains for sporulation phenotypes. Sporulation was significantly affected in 16 out of 37 (43%) tested strains. In addition to expanding the knowledge base on B. subtilis sporulation, our findings suggest that evolutionary age could be used to help with genome mining.
genomic phylostratigraphy
sporulation
Bacillus subtilis
genome mining
Författare
Lei Shi
Chalmers, Biologi och bioteknik, Systembiologi
Abderahmane Derouiche
Chalmers, Biologi och bioteknik, Systembiologi
Santosh Pandit
Chalmers, Biologi och bioteknik, Systembiologi
Shadi Rahimi
Chalmers, Biologi och bioteknik, Systembiologi
Aida Kalantari
Chalmers, Biologi och bioteknik, Systembiologi
Momir Futo
Ruder Boskovic Institute
Vaishnavi Ravikumar
Danmarks Tekniske Universitet (DTU)
Carsten Jers
Danmarks Tekniske Universitet (DTU)
Venkata Raghavendra Subrahmanya Sar Mokkapati
Chalmers, Biologi och bioteknik, Systembiologi
Kristian Vlahovicek
Sveučilište u Zagrebu
Högskolan i Skövde
Ivan Mijakovic
Danmarks Tekniske Universitet (DTU)
Chalmers, Biologi och bioteknik, Systembiologi
Molecular Biology and Evolution
0737-4038 (ISSN) 1537-1719 (eISSN)
Vol. 37 6 1667-1678Ämneskategorier
Evolutionsbiologi
Biokemi och molekylärbiologi
Genetik
DOI
10.1093/molbev/msaa035
PubMed
32061128