Expression of 22 serotonin-related genes in rat brain after sub-acute serotonin depletion or reuptake inhibition
Artikel i vetenskaplig tidskrift, 2020

Objective:
Although the assessment of expression of serotonin-related genes in experimental animals has become a common strategy to shed light on variations in brain serotonergic function, it remains largely unknown to what extent the manipulation of serotonin levels causes detectable changes in gene expression. We therefore chose to investigate how sub-acute depletion or elevation of brain serotonin influences the expression of a number of serotonin-related genes in six brain areas.
Methods:
Male Wistar rats were administered a serotonin synthesis inhibitor, para-chlorophenylalanine (p-CPA), or a serotonin reuptake inhibitor, paroxetine, for 3 days and then sacrificed. The expression of a number of serotonin-related genes in the raphe nuclei, hypothalamus, amygdala, striatum, hippocampus and prefrontal cortex was investigated using real-time quantitative PCR (rt-qPCR).
Results:
While most of the studied genes were uninfluenced by paroxetine treatment, we could observe a robust downregulation of tryptophan hydroxylase-2 in the brain region where the serotonergic cell bodies reside, that is, the raphe nuclei. p-CPA induced a significant increase in the expression of Htr1b and Htr2a in amygdala and of Htr2c in the striatum and a marked reduction in the expression of Htr6 in prefrontal cortex; it also enhanced the expression of the brain-derived neurotrophic factor (Bdnf) in raphe and hippocampus.
Conclusion:
With some notable exceptions, the expression of most of the studied genes is left unchanged by short-term modulation of extracellular levels of serotonin.

Serotonin

prefrontal cortex

hippocampus

SSRI

amygdale

Författare

Jakob Näslund

Göteborgs universitet

Erik Studer

Göteborgs universitet

Staffan Nilsson

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

Elias Eriksson

Göteborgs universitet

Acta Neuropsychiatrica

0924-2708 (ISSN) 1601-5215 (eISSN)

Vol. 32 3 159-165 PII S0924270820000095

Ämneskategorier

Neurovetenskaper

Cell- och molekylärbiologi

Utvecklingsbiologi

DOI

10.1017/neu.2020.9

PubMed

32063244

Mer information

Senast uppdaterat

2020-12-03