Production of 3-hydroxypropionic acid from acetate using metabolically-engineered and glucose-grown Escherichia coli
Artikel i vetenskaplig tidskrift, 2021

Acetate can be used as carbon feedstock for the production of 3-hydroxypropionic acid (3-HP), but the production level was low due to inefficient cell growth on acetate. To better utilize acetate, a two-stage strategy, whereby glucose is used for cell growth and acetate for 3-HP formation, was attempted. Dissected malonyl-CoA reductase of Chloroflexus aurantiacus, alone or along with acetyl-CoA carboxylase and/or transhydrogenase, was overexpressed, and by-products formation pathway, glyoxylate shunt (GS) and gluconeogenesis were modified. When GS or gluconeogenesis was disrupted, cell growth on glucose was not hampered, while on acetate it was completely abolished. Consequently, 3-HP production, at production stage, were low, though 3-HP yield on acetate was increased, especially in the case of aceA deletion. In two-stage bioreactor, strain with upregulated GS produced 7.3 g/L 3-HP with yield of 0.26 mol/mol acetate. This study suggests that two-stage cultivation is a good strategy for 3-HP production from acetate.

Engineered Escherichia coli

3-Hydroxypropionic acid

Two-stage process

Acetate utilization

Malonyl-CoA reductase

Författare

Suman Lama

Ulsan National Institute of Science and Technology (UNIST)

Yeonhee Kim

Ulsan National Institute of Science and Technology (UNIST)

Dat Tuan Nguyen

Ulsan National Institute of Science and Technology (UNIST)

Chaeho Im

Chalmers, Biologi och bioteknik, Industriell bioteknik

Ulsan National Institute of Science and Technology (UNIST)

Mugesh Sankaranarayanan

Vel Tech

Sunghoon Park

Ulsan National Institute of Science and Technology (UNIST)

Bioresource Technology

0960-8524 (ISSN)

Vol. 320 124362

Ämneskategorier

Kemiska processer

Bioprocessteknik

Annan industriell bioteknik

DOI

10.1016/j.biortech.2020.124362

PubMed

33186840

Mer information

Senast uppdaterat

2020-12-15