Investigation of the role of SDHB inactivation in sporadic phaeochromocytoma and neuroblastoma.
Artikel i vetenskaplig tidskrift, 2004

Germline mutations in the succinate dehydrogenase (SDH) (mitochondrial respiratory chain complex II) subunit B gene, SDHB, cause susceptibility to head and neck paraganglioma and phaeochromocytoma. Previously, we did not identify somatic SDHB mutations in sporadic phaeochromocytoma, but SDHB maps to 1p36, a region of frequent loss of heterozygosity (LOH) in neuroblastoma as well. Hence, to evaluate SDHB as a candidate neuroblastoma tumour suppressor gene (TSG) we performed mutation analysis in 46 primary neuroblastomas by direct sequencing, but did not identify germline or somatic SDHB mutations. As TSGs such as RASSF1A are frequently inactivated by promoter region hypermethylation, we designed a methylation-sensitive PCR-based assay to detect SDHB promoter region methylation. In 21% of primary neuroblastomas and 32% of phaeochromocytomas (32%) methylated (and unmethylated) alleles were detected. Although promoter region methylation was also detected in two neuroblastoma cell lines, this was not associated with silencing of SDHB expression, and treatment with a demethylating agent (5-azacytidine) did not increase SDH activity. These findings suggest that although germline SDHB mutations are an important cause of phaeochromocytoma susceptibility, somatic inactivation of SDHB does not have a major role in sporadic neural crest tumours and SDHB is not the target of 1p36 allele loss in neuroblastoma and phaeochromocytoma.

Neural Crest

Molecular Sequence Data

Tumor

Loss of Heterozygosity

DNA Methylation

genetics

Succinate Dehydrogenase

Base Sequence

Iron-Sulfur Proteins

genetics

Promoter Regions (Genetics)

Humans

genetics

Mutation

Gene Silencing

Protein Subunits

Pheochromocytoma

genetics

Cell Line

Neuroblastoma

Författare

D Astuti

M Morris

Cecilia Krona

Göteborgs universitet

Frida Abel

Göteborgs universitet

D Gentle

Tommy Martinsson

Göteborgs universitet

P Kogner

H P H Neumann

R Voutilainen

C Eng

P Rustin

Farida Latif

E R Maher

British Journal of Cancer

0007-0920 (ISSN) 1532-1827 (eISSN)

Vol. 91 1835-41

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1038/sj.bjc.6602202