TIRF Microscopy-Based Monitoring of Drug Permeation Across a Lipid Membrane Supported on Mesoporous Silica
Artikel i vetenskaplig tidskrift, 2020

There is an urgent demand for analytic approaches that enable precise and representative quantification of the transport of biologically active compounds across cellular membranes. In this study, we established a new means to monitor membrane permeation kinetics, using total internal reflection fluorescence microscopy confined to a ≈500 nm thick mesoporous silica substrate, positioned underneath a planar supported cell membrane mimic. This way, we demonstrate spatiotemporally resolved membrane permeation kinetics of a small-molecule model drug, felodipine, while simultaneously controlling the integrity of, and monitoring the drug binding to, the cell membrane mimic. By contrasting the permeation behaviour of pure felodipine with felodipine coupled to the permeability enhancer caprylate (C8), we provide evidence for C8-facilitated transport across lipid membranes, thus validating the potential for this approach to successfully quantify carrier system-induced changes to cellular membrane permeation.

membrane permeation

mesoporous silica

drug delivery

total internal reflection fluorescence

supported lipid bilayer

Författare

Paul Joyce

Chalmers, Fysik, Biologisk fysik

Silver Jõemetsa

Chalmers, Fysik, Biologisk fysik

Simon Isaksson

Chalmers, Fysik, Nano- och biofysik

Shakhawath Hossain

Uppsala universitet

Per Larsson

Uppsala universitet

Christel A.S. Bergström

Uppsala universitet

Fredrik Höök

Chalmers, Fysik, Nano- och biofysik

Angewandte Chemie - International Edition

1433-7851 (ISSN) 1521-3773 (eISSN)

Vol. In Press

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi

Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

DOI

10.1002/anie.202011931

Mer information

Senast uppdaterat

2020-12-17