Metabolic cooperation and spatiotemporal niche partitioning in a kefir microbial community
Artikel i vetenskaplig tidskrift, 2021

Microbial communities often undergo intricate compositional changes yet also maintain stable coexistence of diverse species. The mechanisms underlying long-term coexistence remain unclear as system-wide studies have been largely limited to engineered communities, ex situ adapted cultures or synthetic assemblies. Here, we show how kefir, a natural milk-fermenting community of prokaryotes (predominantly lactic and acetic acid bacteria) and yeasts (family Saccharomycetaceae), realizes stable coexistence through spatiotemporal orchestration of species and metabolite dynamics. During milk fermentation, kefir grains (a polysaccharide matrix synthesized by kefir microorganisms) grow in mass but remain unchanged in composition. In contrast, the milk is colonized in a sequential manner in which early members open the niche for the followers by making available metabolites such as amino acids and lactate. Through metabolomics, transcriptomics and large-scale mapping of inter-species interactions, we show how microorganisms poorly suited for milk survive in—and even dominate—the community, through metabolic cooperation and uneven partitioning between grain and milk. Overall, our findings reveal how inter-species interactions partitioned in space and time lead to stable coexistence.

Författare

Sonja Blasche

European Molecular Biology Laboratory

Yongkyu Kim

European Molecular Biology Laboratory

Ruben A.T. Mars

Eidgenössische Technische Hochschule Zürich (ETH)

Daniel Machado

European Molecular Biology Laboratory

Maria Maansson

Chr. Hansen

Eleni Kafkia

European Molecular Biology Laboratory

University of Cambridge

Alessio Milanese

European Molecular Biology Laboratory

Georg Zeller

European Molecular Biology Laboratory

B. Teusink

Vrije Universiteit Amsterdam

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Vladimir Benes

European Molecular Biology Laboratory

Rute Neves

Chr. Hansen

Uwe H. Sauer

Eidgenössische Technische Hochschule Zürich (ETH)

Kiran Raosaheb Patil

European Molecular Biology Laboratory

University of Cambridge

Nature Microbiology

2058-5276 (eISSN)

Vol. 6 196-208

Ämneskategorier

Botanik

Ekologi

Mikrobiologi

DOI

10.1038/s41564-020-00816-5

PubMed

33398099

Mer information

Senast uppdaterat

2021-02-01