Volume-amplified magnetic bioassay integrated with microfluidic sample handling and high-Tc SQUID magnetic readout
Artikel i vetenskaplig tidskrift, 2018
A bioassay based on a high-Tc superconducting quantum interference device (SQUID) reading out functionalized magnetic nanoparticles (fMNPs) in a prototype microfluidic platform is presented. The target molecule recognition is based on volume amplification using padlock-probe-ligation followed by rolling circle amplification (RCA). The MNPs are functionalized with single-stranded oligonucleotides, which give a specific binding of the MNPs to the large RCA coil product, resulting in a large change in the amplitude of the imaginary part of the ac magnetic susceptibility. The RCA products from amplification of synthetic Vibrio cholera target DNA were investigated using our SQUID ac susceptibility system in microfluidic channel with an equivalent sample volume of 3 μl. From extrapolation of the linear dependence of the SQUID signal versus concentration of the RCA coils, it is found that the projected limit of detection for our system is about 1.0 e5 RCA coils (0.2e−18 mol), which is equivalent to 66 fM in the 3 μl sample volume. This ultra-high magnetic sensitivity and integration with microfluidic sample handling are critical steps towards magnetic bioassays for rapid detection of DNA and RNA targets at the point of care.
POC
high-Tc SQUID
microfluidics
superconductivity
Bioassay
Författare
Sobhan Sepehri
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Emil Eriksson
RISE Research Institutes of Sweden
Alexei Kalaboukhov
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
Teresa Zardán Gómez de la Torre
Uppsala universitet
Kiryl Kustanovich
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Aldo Jesorka
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Justin Schneiderman
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
MedTech West
Jakob Blomgren
RISE Research Institutes of Sweden
Christer Johansson
RISE Research Institutes of Sweden
Maria Strømme
Uppsala universitet
Dag Winkler
Chalmers, Mikroteknologi och nanovetenskap, Kvantkomponentfysik
APL Bioengineering
24732877 (eISSN)
Vol. 2 1 016102Bärbar influensa diagnostic "FLU-ID"
Stiftelsen för Strategisk forskning (SSF) (SBE13-0125), 2021-01-01 -- 2021-03-31.
Stiftelsen för Strategisk forskning (SSF) (SBE13-0125), 2014-06-01 -- 2020-12-31.
Styrkeområden
Nanovetenskap och nanoteknik
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Biokemi och molekylärbiologi
Analytisk kemi
Annan fysik
Medicinsk bioteknik
Nanoteknik
Nanoteknik
Annan industriell bioteknik
Fundament
Grundläggande vetenskaper
Infrastruktur
Nanotekniklaboratoriet
DOI
10.1063/1.4999713