Imbalance of the mitochondrial pro- and anti-apoptotic mediators in neuroblastoma tumours with unfavourable biology.
Artikel i vetenskaplig tidskrift, 2005
It has been proposed that a lack of apoptosis plays an important role in neuroblastoma (NB) progression. We therefore screened cDNA array filters, including 198 apoptotic genes, in order to identify mRNA transcripts that are differentially expressed in tumours with unfavourable versus favourable biology. Twenty-one genes were analysed further using real-time reverse-transcriptase-polymerase chain reaction (RT-PCR). Significantly lower levels of DNCL1 (PIN; P(c)(corrected) = 0.0054) and NTRK1 (TrkA; P(c) = 0.039) were found in NB tumours with unfavourable biology. In addition, BID, BCL2, APAF1, CASP2, CASP3 and CASP9 were found to be preferentially expressed in tumours with favourable biology, whereas CDKN1A (p21), IL2RA, and MCL1, were found to be preferentially expressed in NB tumours with unfavourable biology. In conclusion, mRNA levels of transcripts encoding pro-apoptotic mediators of the mitochondrial apoptotic pathway were found to be expressed to a lower extent in tumours with unfavourable biology. Our data also suggest that the mitochondrial pathway is suppressed in advanced stages of NB tumours, due to an imbalance between anti-apoptotic and pro-apoptotic mediators which is a finding that may have therapeutic significance.
pathology
pathology
genetics
genetics
genetics
genetics
Mitochondria
genetics
genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA
methods
Messenger
RNA Precursors
Neuroblastoma
Apoptosis
DNA
Complementary
Humans
Författare
Frida Abel
Göteborgs universitet
Rose-Marie Sjöberg
Göteborgs universitet
Staffan Nilsson
Chalmers, Matematiska vetenskaper, Matematisk statistik
Göteborgs universitet
Per Kogner
Karolinska universitetssjukhuset
Tommy Martinsson
Göteborgs universitet
European Journal of Cancer
0959-8049 (ISSN)
Vol. 41 4 635-46Ämneskategorier
MEDICIN OCH HÄLSOVETENSKAP
DOI
10.1016/j.ejca.2004.12.021