Quantifying absolute gene expression profiles reveals distinct regulation of central carbon metabolism genes in yeast
Artikel i vetenskaplig tidskrift, 2021

In addition to controlled expression of genes by specific regulatory circuits, the abundance of proteins and transcripts can also be influenced by physiological states of the cell such as growth rate and metabolism. Here we examine the control of gene expression by growth rate and metabolism, by analyzing a multi-omics dataset consisting of absolute-quantitative abundances of the transcriptome, proteome, and amino acids in 22 steady-state yeast cultures. We find that transcription and translation are coordinately controlled by the cell growth rate via RNA polymerase II and ribosome abundance, but they are independently controlled by nitrogen metabolism via amino acid and nucleotide availabilities. Genes in central carbon metabolism, however, are distinctly regulated and do not respond to the cell growth rate or nitrogen metabolism as all other genes. Understanding these effects allows the confounding factors of growth rate and metabolism to be accounted for in gene expression profiling studies.


Rosemary Brown

Chalmers, Fysik, Kemisk fysik

Egor Vorontsov

Göteborgs universitet

C. Sihlbom

Göteborgs universitet

Jens B Nielsen

BioInnovation Institute

Danmarks Tekniske Universitet (DTU)

Chalmers, Biologi och bioteknik, Systembiologi


2050084x (eISSN)

Vol. 10 e65722




Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)





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